| METHOD OF MAINTAINING STATIC ENDURANCE IN DYSFERLIN-DEFICIENT MICE IN EXPERIMENT | |
| 2023-12-26 | |
| 专利权人 | UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) |
| 申请日期 | 2023-12-26 |
| 专利号 | RU2829651-C1 |
| 成果简介 | NOVELTY - Invention relates to cell technologies. Described is a method for maintaining static endurance in dysferlin-deficient mice in experiment, involving use of male dysferlin-deficient mice B6.A/J-Dysfprmd and administering a two-vector adeno-associated virus preparation. A preparation based on adeno-associated virus of serotype 9 is administered intramuscularly (i. m.), carrying a codon-optimized cDNA of the dysferlin gene under the control of a muscle-specific promoter in combination with a chimeric intron to enhance dysferlin expression in dose of 5*1012 units virus with the DYSF gene in amount of 50 mcl in the following muscles of both hind limbs: m. vastus lateralis, m. tibialis anterior, m. gastrocnemius lateralis per animal in total 300 mcl of mice B6.A/J-Dysfprmd at age of 6 months with repeated administration of preparation 1 month after the first administration with assessment of physical performance 3 months after the first administration of the preparation, which is confirmed by the results of the test “Animal retention on a slippery vertical rod”. USE - Molecular biology; virology; biomedicine. ADVANTAGE - Invention can be used to preserve static endurance. 1 cl, 4 tbl, 1 ex |
| IPC 分类号 | C12N-015/12 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/18716 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) |
| 推荐引用方式 GB/T 7714 | KOROKIN M V,POKROVSKII M V,DEYKIN A V,et al. METHOD OF MAINTAINING STATIC ENDURANCE IN DYSFERLIN-DEFICIENT MICE IN EXPERIMENT. RU2829651-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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