| METHOD FOR PRODUCING SECRETOME OF MESENCHYMAL STROMAL CELLS CONTAINING EXOSOME FRACTION | |
| 2023-12-28 | |
| 专利权人 | UNIV STAVROPOL MED (UYST-Non-standard) |
| 申请日期 | 2023-12-28 |
| 专利号 | RU2827355-C1 |
| 成果简介 | NOVELTY - Invention relates to a method of producing a mesenchymal stromal cell (MSC) secretome containing an exosome fraction. Proposed method comprises recovering and culturing mouse adipose tissue-derived MSCs, subsequent detachment of cells using 0.25% trypsin solution and 0.02% Versene solution, three times washing with sterile phosphate buffer solution, centrifugation for 5 minutes at 300 g and room temperature between washes, adding 106 living cells in 500 mcl of phosphate buffer into wells of 24-well plate with incubation at room temperature for 24 hours, after which the supernatant is aspirated, filtered using a syringe filter with pore diameter of 0.2 mcm and centrifuged for 20 minutes at 2,000 g. USE - Molecular biology; cell biology; chemistry; pharmacology. ADVANTAGE - Invention provides developing a new method of producing MSC secretome containing exosomes of high purity and concentration. 1 cl, 4 dwg |
| IPC 分类号 | A61K-035/28 ; C12N-005/0775 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/18646 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV STAVROPOL MED (UYST-Non-standard) |
| 推荐引用方式 GB/T 7714 | DIDENKO N N,BOBRYSHEV D V,DOLGALEV A A,et al. METHOD FOR PRODUCING SECRETOME OF MESENCHYMAL STROMAL CELLS CONTAINING EXOSOME FRACTION. RU2827355-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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