METHOD FOR QUANTITATIVE DETERMINATION OF AMOUNT OF FLAVONOIDS IN GRATIOLE HERB
	FOMINA I A; SHESTOPALOVA N B; KALIUTA T I; FEDONNIKOV A S
	2023-12-28
专利权人	FEDERAL STATE BUDGETARY EDUCATIONAL (BUDG-Non-standard)
申请日期	2023-12-28
专利号	RU2829551-C1
成果简介	NOVELTY - Disclosed is a method for the quantitative determination of the amount of flavonoids in the gratiole herb, involving extraction of raw materials with ethyl alcohol in concentration of 70%, with subsequent determination of the sum of flavonoids by differential spectrophotometry at analytical wavelength 398 nm by complexation reaction for 40 minutes with 5% alcohol solution of aluminium chloride in terms of luteolin-7-glucoside, wherein for the complexation reaction, the ratio of the volumes of extraction from the raw material of the gratiole herb to 5% alcohol solution of aluminium chloride is 1:2, to obtain an aqueous-alcoholic extract from gratiole herb, extraction is carried out in a boiling water bath for 60 minutes, the raw material is preliminarily prepared by grinding gratiole herb to 2 mm, when using 100 ml of ethyl alcohol in concentration of 70% per 1 g of the raw material weighed amount, the content of the sum of flavonoids in terms of luteolin-7-glucoside and absolutely dry raw material is calculated. USE - Chemical-pharmaceutical industry. ADVANTAGE - Invention provides the development of a method for quantitative determination of the sum of flavonoids in the gratiole herb, having high specificity and accuracy due to processing of multidimensional spectral data. 1 cl, 4 dwg, 5 tbl, 2 ex
IPC 分类号	B01D-011/02 ; G01N-001/40 ; G01N-021/31
国家	俄罗斯
专业领域	化学化工
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/18645
专题	中国科学院新疆生态与地理研究所
作者单位	FEDERAL STATE BUDGETARY EDUCATIONAL (BUDG-Non-standard)
推荐引用方式 GB/T 7714	FOMINA I A,SHESTOPALOVA N B,KALIUTA T I,et al. METHOD FOR QUANTITATIVE DETERMINATION OF AMOUNT OF FLAVONOIDS IN GRATIOLE HERB. RU2829551-C1[P]. 2023.

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