| RECOMBINANT PLASMID pNhaDAAO, PROVIDING SYNTHESIS OF OXIDASE OF COMMON AND N-SUBSTITUTED D-AMINO ACIDS (NhaDAAO) OF ARCHAEA NATRARCHAEOBIUS HALALKALIPHILUS AArcht4 (NhaDAAO) IN ESCHERICHIA COLI CELLS, AND RECOMBINANT STRAIN ESCHERICHIA COLI BL21 (DE3)/pNhaDAAO VKPM B-14556 - PRODUCER NhaDAAO | |
| 2023-12-28 | |
| 专利权人 | UNIV MOSC LOMONOSOV (MOSU-C) |
| 申请日期 | 2023-12-28 |
| 专利号 | RU2829362-C1 |
| 成果简介 | NOVELTY - Invention relates to biotechnology, particularly to genetic engineering, and represents recombinant plasmid DNA pNhaDAAO containing a modified Nhadaao gene coding D-amino acid oxidase Natrarchaeobius halalkaliphilus archaea AArcht4 (NhaDAAO). Obtained plasmid provides expression of the modified gene included in its nucleotide sequence in Escherichia coli cells. As a result of transformation of the strain E. coli BL21 (DE3) with the proposed plasmid, a new recombinant producer strain E. coli BL21 (DE3)/pNhaDAAO VKPM B-14556 is obtained, which provides expression of NhaDAAO in an active form. USE - Biotechnology. ADVANTAGE - Invention enables to obtain NhaDAAO in an active form with high efficiency. 2 cl, 4 dwg, 5 tbl, 4 ex |
| IPC 分类号 | C12N-001/21 ; C12N-015/11 ; C12N-015/53 ; C12N-015/70 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/18615 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV MOSC LOMONOSOV (MOSU-C) |
| 推荐引用方式 GB/T 7714 | TISHKOV V I,ATROSHENKO D L,GOLOVINA D I,et al. RECOMBINANT PLASMID pNhaDAAO, PROVIDING SYNTHESIS OF OXIDASE OF COMMON AND N-SUBSTITUTED D-AMINO ACIDS (NhaDAAO) OF ARCHAEA NATRARCHAEOBIUS HALALKALIPHILUS AArcht4 (NhaDAAO) IN ESCHERICHIA COLI CELLS, AND RECOMBINANT STRAIN ESCHERICHIA COLI BL21 (DE3)/pNhaDAAO VKPM B-14556 - PRODUCER NhaDAAO. RU2829362-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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