| RECOMBINANT PLASMID pHpFDH_HisA, PROVIDING SYNTHESIS IN ESCHERICHIA coli CELLS OF FORMATE DEHYDROGENASE OF METHYLOTROPHIC YEAST HANSENULA POLYMORPHA (HpFDH_HisA) AND RECOMBINANT STRAIN ESCHERICHIA coli BL21(DE3)/pHpFDH_HisA VKPM B-14559 - HpFDH_HisA PRODUCER | |
| 2023-12-28 | |
| 专利权人 | UNIV MOSC LOMONOSOV (MOSU-C) |
| 申请日期 | 2023-12-28 |
| 专利号 | RU2829762-C1 |
| 成果简介 | NOVELTY - Invention relates to biotechnology, particularly to genetic engineering. Was constructed recombinant plasmid DNA pHpFDH_HisA, containing modified gene hpfdh_hisa, coding mutant `NAD+-dependent formate dehydrogenase (EC 1.2.1.2, HpFDH_HisA) with additional sequence of six histidine residues and one residue of alanine on N-terminus of enzyme. Addition of an additional sequence of His6Ala to the N-terminus of the enzyme increases thermal stability and enables to obtain preparations of a highly purified enzyme in a single step. To ensure high efficiency of expression in the target producer strain in the modified HpFDH_HisA gene, a TAATAA triplet tandem is used as a stop codon instead of the TGA triplet in the initial gene. Obtained plasmid enables expression of the included gene hpfdh_hisa in Escherichia coli cells. USE - Biotechnology. ADVANTAGE - As a result of transformation of the Escherichia coli BL21(DE3) strain by the proposed plasmid, a new recombinant producer strain is obtained Escherichia coli BL21(DE3))/pHpFDH_HisA VKPM B-14559, providing overexpression of HpFDH_HisA in active form. 2 cl, 6 dwg, 4 tbl, 4 ex |
| IPC 分类号 | C12N-001/21 ; C12N-015/11 ; C12N-015/53 ; C12N-015/70 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/18614 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV MOSC LOMONOSOV (MOSU-C) |
| 推荐引用方式 GB/T 7714 | TISHKOV V I,POMETUN A A,SHAPOSHNIKOV L A,et al. RECOMBINANT PLASMID pHpFDH_HisA, PROVIDING SYNTHESIS IN ESCHERICHIA coli CELLS OF FORMATE DEHYDROGENASE OF METHYLOTROPHIC YEAST HANSENULA POLYMORPHA (HpFDH_HisA) AND RECOMBINANT STRAIN ESCHERICHIA coli BL21(DE3)/pHpFDH_HisA VKPM B-14559 - HpFDH_HisA PRODUCER. RU2829762-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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