| OPTIMIZED STRAIN OF POLIOVIRUS TYPE 3 FOR THERAPY OF SOLID TUMOURS | |
| 2023-12-29 | |
| 专利权人 | ENGELHARDT INST MOLECULAR BIOLOGY (ENGE-Non-standard) ; FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-12-29 |
| 专利号 | RU2836222-C1 |
| 成果简介 | NOVELTY - Described is a method for optimizing Sabin type 3 poliovirus vaccine for selective destruction of tumour cells, involving: culturing Sabin type 3 poliovirus vaccine on a mixture of glioblastoma tumour cells, wherein replication is initiated in the HEK293T cell culture, 24 hours after infection, the cells are washed and incubated for 48 hours, after which the virus is isolated from the culture; virus passaging for six months in a mixed culture of glioblastoma cells with determination of virus titre and analysis of its cytopathic properties; modification of internal ribosome binding site (IRES), by amplification of IRES using primers SEQ ID NO: 1 and SEQ ID NO: 2, amplification of a DNA fragment coding a genome of a plasmid backbone using primers SEQ ID NO: 3 and SEQ ID NO: 4, with subsequent creation of circular plasmid, which is induced by virus. Invention enables to obtain a highly oncolytic recombinant poliovirus which effectively lyses solid tumours of various histological affiliations. Strain obtained using the disclosed method has shown its effectiveness and safety in experiments in vitro on models of tumour and conditionally normal cell cultures. USE - Biotechnology. ADVANTAGE - Optimized recombinant strain has shown high oncolytic activity on various models in vivo in immunodeficient mice with xenografts of human tumours and humanized model of orthotopic rat C6 glioma. 2 cl, 4 dwg, 6 ex |
| IPC 分类号 | C12N-005/10 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/18594 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | 1.ENGELHARDT INST MOLECULAR BIOLOGY (ENGE-Non-standard) 2.FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | LIPATOVA A V,VOROBEV P O,KUPRASH D V,et al. OPTIMIZED STRAIN OF POLIOVIRUS TYPE 3 FOR THERAPY OF SOLID TUMOURS. RU2836222-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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