| METHOD FOR DETERMINING TYLOSIN IN OBJECTS OF VETERINARY SURVEILLANCE | |
| 2024-01-12 | |
| 专利权人 | SUPERHARD & NEW CARBON MATERIALS INST (SUPE-Non-standard) |
| 申请日期 | 2024-01-12 |
| 专利号 | RU2827514-C1 |
| 成果简介 | NOVELTY - Method of determining tylosin in objects of veterinary surveillance – products of animal origin or fodders – consists in the fact that a sample is homogenised, centrifuged, the supernatant is separated and tylosin is determined by enzyme immunoassay, wherein to the sample of the object of veterinary surveillance after homogenisation acetonitrile is added in a weight ratio of sample : acetonitrile equal to 1:3.5–5.0, the reaction mixture is stirred at room temperature for 20–30 minutes, centrifuged at 2,000–2,500 rpm for 5–10 minutes with subsequent separation of supernatant, further, it is evaporated in a stream of nitrogen to 10.0–15.0% of the initial volume of the sample and bidistilled water is added in a weight ratio of sample : water equal to 1:2.0–5.0, and continuing to evaporate in a stream of nitrogen until evaporation of acetonitrile with subsequent introduction into the reaction mixture of n-hexane in a weight ratio of sample : n-hexane equal to 1:3.5–5.0, reaction mixture is stirred at room temperature for 20–30 minutes, centrifuged at 2,000–2,500 rpm for 5–10 minutes with subsequent removal of upper fat layer in n-hexane, and water supernatant is used for enzyme immunoassay. USE - Veterinary science. ADVANTAGE - Invention increases accuracy of determining tylosin in objects of veterinary surveillance. 1 cl, 9 ex |
| IPC 分类号 | A61K-031/7048 ; G01N-033/00 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/18442 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | SUPERHARD & NEW CARBON MATERIALS INST (SUPE-Non-standard) |
| 推荐引用方式 GB/T 7714 | SATIUKOVA L P,POPOV PY A,BELOUSOV V I,et al. METHOD FOR DETERMINING TYLOSIN IN OBJECTS OF VETERINARY SURVEILLANCE. RU2827514-C1[P]. 2024. |
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