Micropropagation system for enhanced strawberry plant multiplication, comprises surface sterilization chamber comprising ultraviolet ray lamps to sterilize strawberry runner tip explants, and sterile culture generation chamber to culture sterilized explants in controlled culture room
	SAHOO S; SAHOO K K
	2024-01-16
专利权人	SAHOO S (SAHO-Individual) ; SAHOO K K (SAHO-Individual)
申请日期	2024-01-16
专利号	IN202431003139-A
成果简介	NOVELTY - Micropropagation system (100) comprises a surface sterilization chamber (102) including one or more ultraviolet ray lamps to sterilize strawberry runner tip explants, a sterile culture generation chamber (104) to culture the sterilized explants in a controlled culture room, where temperature is maintained between 22℃ to 23℃ and a photoperiod of 12 hours a day. A multiple shoot regeneration chamber (106) is set for inducing shoot regeneration in the explants, a root initiation chamber (108) is set for inducing root formation in the regenerated shoots, and an acclimatization chamber is used for acclimatizing the rooted plantlets to ex vitro conditions. USE - Micropropagation system for enhanced strawberry plant multiplication and tissue culture. ADVANTAGE - The micropropagation system ensure uniform light exposure and nutrient absorption from all directions, and promote even and accelerated strawberry plant shoot development. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method of micropropagation, involves: (1) carrying out surface sterilizing runner shoot tips of strawberry plants; (2) preparing sterile culture medium comprising modified Murashige and Skoog (MS) basal medium, 3 wt.% sucrose, and 0.8 wt.% agar; (3) culturing the sterilized runner shoot tips obtained in step (1) in the sterile culture mediumprepared in step (2) in a controlled tissue culture room maintained at a temperature of 22℃-23℃ with a photoperiod of 12 hours a day for 1 week; (4) preparing a multiple shoot regenerating medium comprising modified MS basal medium, 3 wt.% sucrose, 0.8 wt.% agar, and 2 mg/L metatopollin growth hormone; (5) culturing and subculturing the sterilized runner shoot tips obtained in step (3) in the multiple shoots regenerating medium prepared in step (4) an initial inoculation period of 2 weeks, then subculturing after 1 week in the same medium, and conducting in a controlled tissue culture room at a temperature of 22℃-23℃ with a photoperiod of 12 hours a day; (6) preparing a rooting medium comprising 1/2 MS basal medium, 3 wt.% sucrose, and 0.6 wt.% agar; and (7) transferring the growing shoots obtained in step (5) into the rooting medium prepared in step (6) by placing the growing shoots into the rooting medium for 3 weeks in a controlled room at a temperature of 22℃-23℃ with a photoperiod of 12 hours a day, and achieving a root regeneration frequency of 95-99%. DESCRIPTION OF DRAWING(S) - The drawing shows a block diagram of micropropagation system. 100Micropropagation system 102Surface sterilization chamber 104Sterile culture generation chamber 106Shoot regeneration chamber 108Root initiation chamber
IPC 分类号	A01G-022/05 ; A01G-025/16 ; A01G-007/00 ; A01G-009/20 ; A01G-009/24 ; A01H-004/00
国家	印度
专业领域	化学化工
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/18420
专题	中国科学院新疆生态与地理研究所
作者单位	1.SAHOO S (SAHO-Individual) 2.SAHOO K K (SAHO-Individual)
推荐引用方式 GB/T 7714	SAHOO S,SAHOO K K. Micropropagation system for enhanced strawberry plant multiplication, comprises surface sterilization chamber comprising ultraviolet ray lamps to sterilize strawberry runner tip explants, and sterile culture generation chamber to culture sterilized explants in controlled culture room. IN202431003139-A[P]. 2024.

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