OPTIMIZED RELEASE OF ENDOGENOUS BETA-DEFENSINS
	BAZIKOV I A; MALTSEV A N; EFREMENKO A A; BAZIKOV F I
	2024-01-24
专利权人	UNIV STAVROPOL MED (UYST-Non-standard) ; BAZIKOV I A (BAZI-Individual)
申请日期	2024-01-24
专利号	RU2825572-C1
成果简介	NOVELTY - Invention relates to medical biotechnology, specifically to release of endogenous beta-defensins from placental tissue. Disclosed is a method of separating beta-defensins from placental tissue cells, involving grinding, clarification with hydrogen peroxide solution, homogenisation in three steps: mechanical grinding, ultrasonic action with diameter of used nozzle 10 mm and increase of rotation speed to 2,000 rpm, time up to 30 minutes at temperature 4 °C, enzymatic hydrolysis of placental tissue cells with 2% trypsin on a magnetic stirrer, correction of the medium with Versene solution, cell sedimentation by centrifugation at 10,000 g, extraction of peptides on a separation column, with pre-ionisation of Sephadex G-25 by passing through the plates of direct current with force of 5 mA while passing the hydrolyzate through the Sephadex G-25 layer, followed by washing out beta-defensins from Sephadex G-25 particles with a cold Hanks solution, sterilizing filtration through fine-pore filters with pore diameter of 0.2 mcm and virus inactivation with acetic acid. USE - Medicine; biotechnology. ADVANTAGE - Invention provides optimizing the recovery of the fraction of endogenous beta-defensins with high biological value for further development of pharmaceutical compositions. 1 cl, 1 dwg, 1 tbl, 1 ex
IPC 分类号	C07K-001/14 ; C07K-001/16 ; C07K-001/36
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/18351
专题	中国科学院新疆生态与地理研究所
作者单位	1.UNIV STAVROPOL MED (UYST-Non-standard) 2.BAZIKOV I A (BAZI-Individual)
推荐引用方式 GB/T 7714	BAZIKOV I A,MALTSEV A N,EFREMENKO A A,et al. OPTIMIZED RELEASE OF ENDOGENOUS BETA-DEFENSINS. RU2825572-C1[P]. 2024.

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