METHOD OF PRODUCING PROTEIN-PHOSPHATE COMPLEX FROM BEANS, HAVING INHIBITORY ACTIVITY ON -AMYLASES
	BAZULEVA V A; PRUTENSKAYA E A; USHCHAPOVSKII V I; SULMAN M G
	2024-02-09
专利权人	UNIV TVER STATE TECH (UYTV-Non-standard)
申请日期	2024-02-09
专利号	RU2828662-C1
成果简介	NOVELTY - Invention relates to biotechnology, namely to a method of producing a protein-phosphate complex – inhibitors α-amylase from leguminous crops. Method of producing protein-phosphate complex of beans, having inhibitory activity on α-amylase, including milling beans in a mill to flour state, sieving through a sieve with a hole diameter of 1 mm, extraction of protein substances in an ultrasonic bath with parameters of 95 V, 50/60 Hz using a phosphate buffer solution with pH 8 for 15 minutes at a ratio of bean flour : buffer solution of 1:15, centrifugation at 4,000 rpm at temperature of 5 °C for 5 minutes, vacuum filtration, precipitation of protein-phosphate complex with cooled acetone in ratio 1:2, secondary vacuum filtration, washing with cooled diethyl ether in amount of 7.5 ml per 1 g of raw material, drying of protein-phosphate complex. USE - Biotechnology. ADVANTAGE - Method described above maximizes output of the end product – protein-phosphate complex and preservation of its biological activity. 1 cl, 1 tbl, 19 ex
IPC 分类号	A61K-036/48 ; C07K-001/02 ; C07K-001/14
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/18196
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV TVER STATE TECH (UYTV-Non-standard)
推荐引用方式 GB/T 7714	BAZULEVA V A,PRUTENSKAYA E A,USHCHAPOVSKII V I,et al. METHOD OF PRODUCING PROTEIN-PHOSPHATE COMPLEX FROM BEANS, HAVING INHIBITORY ACTIVITY ON -AMYLASES. RU2828662-C1[P]. 2024.

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