METHOD FOR ASSESSING MORPHOLOGICAL STRUCTURE OF BIOFILMS OF MICROORGANISMS IN DYNAMICS OF BIOFILM FORMATION
	DMITRY K; KROPOTOV V S
	2024-02-12
专利权人	NIZHNY NOVGOROD EPIDEMIOLOGY & MICROBIOL (NIZH-Non-standard)
申请日期	2024-02-12
专利号	RU2826880-C1
成果简介	NOVELTY - Disclosed is a method for assessing the morphological structure of biofilms of microorganisms, including the formation of a biofilm of microorganisms on a cover glass, for which 50–200 mcl of suspension of mother culture of bacteria, diluted to concentration of 0.5–3.0 by McFarland, applied on a cover glass, incubated for 1 h at 37±2 °C, then the glasses are washed with phosphate buffered saline, immersed with a narrow face in a dense nutrient medium at angle of 40–50° to surface, 10 ml of a liquid nutrient medium is poured into a dish, incubated for 7 days at 37±2 °C with complete replacement of liquid nutrient medium every 24 hours; before staining, the preparation is fixed for 3 minutes in 96% ethanol with subsequent removal of alcohol and gluing with a transparent varnish on a slide, stained with a dye for luminescent microscopy. Structure of the biofilm is visualized using an optical microscope with a luminescent module at magnification x10-100 in an immersion system, and a morphometric study is carried out using the ImageJ ver. 1.52a program. USE - Biotechnology. ADVANTAGE - Invention provides evaluation of morphology of biofilm of microorganisms at different stages of its formation, creation of three-dimensional models with saving of research results in the form of graphic files. 1 cl, 3 dwg, 3 ex
IPC 分类号	C12N-011/00 ; C12Q-001/02 ; G01N-033/483
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/18173
专题	中国科学院新疆生态与地理研究所
作者单位	NIZHNY NOVGOROD EPIDEMIOLOGY & MICROBIOL (NIZH-Non-standard)
推荐引用方式 GB/T 7714	DMITRY K,KROPOTOV V S. METHOD FOR ASSESSING MORPHOLOGICAL STRUCTURE OF BIOFILMS OF MICROORGANISMS IN DYNAMICS OF BIOFILM FORMATION. RU2826880-C1[P]. 2024.

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