Producing hyperactive amyloglucosidase involves preparing an induced Aspergillus niger culture by incubation, creating a substrate mixture by mixing wheat bran or oat bran, Czapek-Dox media, and whey protein, autoclaving substrate mixture and inoculating autoclaved substrate with induced inoculum
	DUTTA S; RAJLAKSHMI; JAYASEELAN P; SARAVANABHUPATHY S; ANUSHA; DEY S; BANERJEE S; SANTRA S; DAS M; BANERJEE R
	2024-02-27
专利权人	INDIAN INST TECHNOLOGY KHARAGPUR (IIKH-C)
申请日期	2024-02-27
专利号	IN202431014189-A
成果简介	NOVELTY - Producing hyperactive amyloglucosidase involves: (a) preparing an induced Aspergillus niger culture with an incubation duration ranging from 60 hours to 170 hours; (b) creating a substrate mixture by mixing wheat bran or oat bran, Czapek-Dox media, and whey protein in a ratio ranging from 1:1:1 to 4:2:1; (c) autoclaving the substrate mixture at any temperature ranging from 117℃ to 125℃ for a period of 30 minutes; (d) inoculating the autoclaved substrate with induced inoculum of Aspergillus niger culture at a maximum concentration of 10 wt.%/vol.%; (e) incubating the inoculated substrate at a temperature ranging from 15℃ to 45℃ for a period of 60 hours to 170 hours for producing fermented biomass; (f) mixing the fermented biomass obtained after incubation with distilled water in a ratio of 1:4; (g) incubating the mixture of fermented biomass and distilled water for a period of 2 hours at a room temperature. USE - Method for producing hyperactive amyloglucosidase. ADVANTAGE - The method is safe, biodegradable and environment friendly. The produced amyloglucosidase has an improved half-life and shelf life extended to more than 24 months at 4℃ making it suitable for long-term applications. The enzyme activity in the hyperactive amyloglucosidase is quantified using a spectrophotometric assay. DETAILED DESCRIPTION - Producing hyperactive amyloglucosidase involves: (a) preparing an induced Aspergillus niger culture with an incubation duration ranging from 60 hours to 170 hours; (b) creating a substrate mixture by mixing wheat bran or oat bran, Czapek-Dox media, and whey protein in a ratio ranging from 1:1:1 to 4:2:1; (c) autoclaving the substrate mixture at any temperature ranging from 117℃ to 125℃ for a period of 30 minutes; (d) inoculating the autoclaved substrate with induced inoculum of Aspergillus niger culture at a maximum concentration of 10 wt.%/vol.%; (e) incubating the inoculated substrate at a temperature ranging from 15℃ to 45℃ for a period of 60 hours to 170 hours for producing fermented biomass; (f) mixing the fermented biomass obtained after incubation with distilled water in a ratio of 1:4; (g) incubating the mixture of fermented biomass and distilled water for a period of 2 hours at a room temperature; (h) extracting the enzyme by filtering the mixture through cheesecloth; and (i) centrifuging the filtrate enzyme to obtain the hyperactive amyloglucosidase.
IPC 分类号	C12N-001/14 ; C12N-009/24
国家	印度
专业领域	化学化工
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/17980
专题	中国科学院新疆生态与地理研究所
作者单位	INDIAN INST TECHNOLOGY KHARAGPUR (IIKH-C)
推荐引用方式 GB/T 7714	DUTTA S,RAJLAKSHMI,JAYASEELAN P,et al. Producing hyperactive amyloglucosidase involves preparing an induced Aspergillus niger culture by incubation, creating a substrate mixture by mixing wheat bran or oat bran, Czapek-Dox media, and whey protein, autoclaving substrate mixture and inoculating autoclaved substrate with induced inoculum. IN202431014189-A[P]. 2024.

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