METHOD FOR STIMULATING LIPOLYTIC ACTIVITY OF LACTOCOCCUS LACTIS BACTERIA
	CHERNOVA A P; PUKHNIARSKAIA D S; SHESTAKOVA A V
	2024-02-29
专利权人	UNIV TOMSK POLYTECHNIC (UYTO-C)
申请日期	2024-02-29
专利号	RU2830250-C1
成果简介	NOVELTY - Disclosed is a method for stimulating the lipolytic activity of Lactococcus lactis bacteria, characterized by the fact that Lactococcus lactis bacteria with density of 108 CFU/ml are cultured on a liquid nutrient medium MRS for 24 hours at temperature of 37 °C, bacterial biomass is separated by centrifugation, the supernatant is sterilized by passing through a membrane filter, and a culture liquid is obtained, 7% of olive oil of the total volume of mixture and 6% of a phosphate buffer with pH 7.0 of the total volume of the mixture are mixed separately, shaken, further, 8% of said culture fluid of the total volume of the mixture and a weighed portion of lithium pyruvate powder are added to the obtained mixture to obtain a solution concentration of 1.5-20 mmol/l, then shaken, incubated for 2 hours at temperature of 37 °C and 96% ethyl alcohol is added in amount of 79% of the total volume of the mixture. USE - Biotechnology. ADVANTAGE - Invention provides higher lipolytic activity of Lactococcus lactis bacteria. 3 cl, 1 dwg, 4 ex
IPC 分类号	A23C-019/00 ; C12N-001/20 ; C12N-001/38 ; C12N-009/20
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/17943
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV TOMSK POLYTECHNIC (UYTO-C)
推荐引用方式 GB/T 7714	CHERNOVA A P,PUKHNIARSKAIA D S,SHESTAKOVA A V. METHOD FOR STIMULATING LIPOLYTIC ACTIVITY OF LACTOCOCCUS LACTIS BACTERIA. RU2830250-C1[P]. 2024.

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