Isolating exosomes from sample, including plant or animal cells, comprises obtaining pre-processed isolation reagent, collecting liquid fraction, adding pre-processed isolation reagent to liquid fraction, centrifuging reaction mixture, and resuspending pellet in buffer solution to isolate exosome
	PAUL S; DUTTA A
	2024-02-29
专利权人	EXSURE PRIVATE LTD (EXSU-Non-standard)
申请日期	2024-02-29
专利号	IN202431014971-A
成果简介	NOVELTY - Isolating exosomes from a sample comprises: (a) obtaining a pre-processed isolation reagent in a pH range between 6.5-7.0, where the pre-processing involves (i) intermittent sonication of the isolation reagent; (ii) extracting a supernatant from the sonicated isolation reagent; and (iii) filtering the supernatant to obtain the pre-processed isolation reagent; (b) collecting a liquid fraction from the sample for isolating the exosomes; (c) adding the pre-processed isolation reagent to the liquid fraction to obtain reaction mixture; (d) centrifuging the reaction mixture to obtain a pellet; and (e) resuspending the pellet in a buffer solution to isolate the exosome, where the exosome obtained is of high purity range between 60-95%. USE - Method for isolating exosomes from a sample, including microbial cell, plant cells and animal cells (claimed). ADVANTAGE - The method increases exosomes yield exhibiting a high degree (95%) purity, and isolates exosomes that are free from cellular debris, micro vesicles, and apoptotic bodies.
IPC 分类号	B01D-015/34 ; C12N-005/00 ; C12N-005/071 ; C12N-005/0775
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/17933
专题	中国科学院新疆生态与地理研究所
作者单位	EXSURE PRIVATE LTD (EXSU-Non-standard)
推荐引用方式 GB/T 7714	PAUL S,DUTTA A. Isolating exosomes from sample, including plant or animal cells, comprises obtaining pre-processed isolation reagent, collecting liquid fraction, adding pre-processed isolation reagent to liquid fraction, centrifuging reaction mixture, and resuspending pellet in buffer solution to isolate exosome. IN202431014971-A[P]. 2024.

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