Three dimensional cell culture device for real-time monitoring of cell viability, comprises a cellulose derived substrate including polymer coated glossy side facilitating capillary action arrested immobilization and uniform distribution of cells in cell culture well bonded to other non-glossy side
	DAS S; CHAKRABORTY S; DHAR D; ANJALI
	2024-03-08
专利权人	INDIAN INST TECHNOLOGY KHARAGPUR (IIKH-C)
申请日期	2024-03-08
专利号	IN202431016852-A
成果简介	NOVELTY - Three dimensional (3D) cell culture device comprises a cellulose derived substrate including a polymer coated glossy side facilitating capillary action arrested immobilization and uniform distribution of cells in cell culture well bonded to the other non-glossy side, where the cellulose derived substrate at its glossy side supported on a glass substrate with its non-glossy side supporting the cell culture well having integrated operating electrodes and operative connect through contact pads. USE - Three dimensional cell culture device for real-time monitoring of cell viability and used in tissue engineering and modeling, replicate disease mechanism, real-time and non-invasive impedimetric monitoring of cell proliferation, cell based assay, development of healthcare diagnostic tools, and cancer aggressiveness. ADVANTAGE - The three-dimensional cell culture device provides cost-effective fabrication of polymer-coated cellulosic substrate enabled 3D cell culture platform having minimal wicking capacity, capillary action and effective cellular adhesion with minimized risk of contamination advantageously free of pre-treatment, functionalization, or patterning of the substrate. The cell culture device is a ready-to-use device, requiring no pre-treatment, functionalization, or patterning and it is favorable for long-term device storage and shelf life. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method of fabrication of 3D cell culture device for real-time monitoring of cell viability, which involves: (i) providing a cellulose derived substrate with a polymer coated glossy side and a non-glossy other side; (ii) providing the glossy side of the cellulose derived substrate supported on a glass substrate with its non-glossy side supporting cell culture well; and (iii) integrating operating electrodes and connecting contact pads on the the nonglossy side supporting cell culture well to enable desired real-time monitoring of cell viability.
IPC 分类号	C12M-001/00 ; C12M-001/12 ; C12M-001/34 ; C12M-003/06 ; C12Q-001/6851
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/17807
专题	中国科学院新疆生态与地理研究所
作者单位	INDIAN INST TECHNOLOGY KHARAGPUR (IIKH-C)
推荐引用方式 GB/T 7714	DAS S,CHAKRABORTY S,DHAR D,et al. Three dimensional cell culture device for real-time monitoring of cell viability, comprises a cellulose derived substrate including polymer coated glossy side facilitating capillary action arrested immobilization and uniform distribution of cells in cell culture well bonded to other non-glossy side. IN202431016852-A[P]. 2024.

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