METHOD FOR SAMPLE PREPARATION FOR AMINO ACID ANALYSIS IN CONDITIONS OF REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
	SUKHIKH A S; LE V M; TROTSENKO N A; ZAKHAROVA YU V
	2024-03-19
专利权人	UNIV KEMEROVO (UYKE-Non-standard)
申请日期	2024-03-19
专利号	RU2829449-C1
成果简介	NOVELTY - Disclosed is a method for preparing samples for amino acid analysis under conditions of reversed-phase high-performance liquid chromatography, involving obtaining an amino acid hydrolysate using 6 M hydrochloric acid solution, derivatization with phenyl isothiocyanate using a chromatographic column, where hydrolysis of a sample of protein or peptide nature is carried out with microwave irradiation at temperature of 150-160 °C for 25-55 minutes with a microwave radiation power of 1,800 W at frequency of 2,450 MHz and pressure of 2.8-3.5 atm, the hydrolysate is dried in a vacuum, sodium hydroxide solution 1 M is added to pH=7.5-7.8, mixed, derivatization is carried out for 30 minutes at temperature of 25 °C with a solution of phenyl isothiocyanate in a mixture of isopropyl alcohol-dimethylsulfoxide, taken in a volume ratio of 1:1. USE - Medicine. ADVANTAGE - Invention increases sensitivity of the derivatizing reagent while simplifying sample preparation. 2 cl, 3 dwg, 2 ex
IPC 分类号	C07B-063/02 ; G01N-001/44 ; G01N-030/06
国家	俄罗斯
专业领域	化学化工
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/17632
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV KEMEROVO (UYKE-Non-standard)
推荐引用方式 GB/T 7714	SUKHIKH A S,LE V M,TROTSENKO N A,et al. METHOD FOR SAMPLE PREPARATION FOR AMINO ACID ANALYSIS IN CONDITIONS OF REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. RU2829449-C1[P]. 2024.

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