Thermoresponsive biomaterial e.g. hydrogel, comprises crosslinked chitosan obtained by crosslinking beta-glycerophosphate and sodium bicarbonate, collagen which provides adhesive surface for cell attachment, and hydroxy apatite nanoparticles which provides mechanical strength to hydrogel
	BHATTACHARYA N; DAVE J; TOMAR G
	2024-03-30
专利权人	UNIV SAVITRIBAI PHULE PUNE (UYSA-Non-standard)
申请日期	2024-03-30
专利号	IN202421026474-A
成果简介	NOVELTY - A thermoresponsive biomaterial comprises a crosslinked chitosan, where the chitosan is crosslinked by electrostatic interaction between 7.5-10 wt./vol.% β-glycerophosphate and 0.1 M sodium bicarbonate, 0.16-2 wt./vol.% collagen which provides adhesive surface for the cell attachment, and 0.05-1 wt./vol.% hydroxyapatite nanoparticles which provides mechanical strength to the hydrogel. USE - Thermoresponsive biomaterial e.g. colloidal gel, organogel or hydrogel for preparing scaffold or filling material for regenerating bone or cartilage tissue in intervertebral disc injuries and ectopic bone formation, and providing support to the damaged tissues (all claimed), treating skin wounds, and use in ink for three-dimensional (3D) printer. No biological data given. ADVANTAGE - The biomaterial is administered easily without the requirement of further medical invasive procedures or equipment, and ready to use, and generates conducive microenvironment for growth and survival of indigenous cells, and facilitates fast tissue regeneration. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method of preparing thermoresponsive biomaterial, which comprises: (a) dissolving 2% chitosan solution in 0.1% acetic acid to obtain homogenous mixture; (b) autoclaving the homogenous mixture at 121℃, 15 psi for 15 minutes; (c) storing the mixture at 4℃ for 12 hours before further processing; (d) autoclaving hydroxy apatite nanoparticles at 121℃, 15 psi for 15 minutes; (e) sterilizing collagen under UV for 2 hours; (f) dissolving glycerophosphate in distilled water containing 0.1 M sodium bicarbonate filter, sterilizing and storing at 4℃ for minimum 2 hours before use; (g) mixing chitosan mixture, hydroxy apatite, and collagen under sterile conditions at 4℃ to form homogenous mixture; and (h) adding glycerophosphate solution dropwise and mixing for at 4℃ 1 hour to obtain thermoresponsive gel.
IPC 分类号	A61K-035/33 ; A61K-035/44 ; A61L-027/10 ; A61L-027/12 ; A61L-027/20 ; A61L-027/22 ; A61L-027/24 ; A61L-027/36
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/17450
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV SAVITRIBAI PHULE PUNE (UYSA-Non-standard)
推荐引用方式 GB/T 7714	BHATTACHARYA N,DAVE J,TOMAR G. Thermoresponsive biomaterial e.g. hydrogel, comprises crosslinked chitosan obtained by crosslinking beta-glycerophosphate and sodium bicarbonate, collagen which provides adhesive surface for cell attachment, and hydroxy apatite nanoparticles which provides mechanical strength to hydrogel. IN202421026474-A[P]. 2024.

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