| Constructing and expressing a recombinant protective antigen (PA) involves gene cloning protective antigen PA63 structural in DNA plasmid, and using DNA chimeras bearing a set of signal sequences targets protective antigen PA63 to subcellular locations | |
| 2024-03-30 | |
| 专利权人 | SUKINDER P S (SUKI-Individual) ; HARSHIL K (HARS-Individual) ; KHANSAHAB H M S (KHAN-Individual) ; NILESHBHAI N (NILE-Individual) ; CHODAVADIYA C (CHOD-Individual) ; CHAUHAN D (CHAU-Individual) ; PAREKH V (PARE-Individual) ; SINGH C (SING-Individual) |
| 申请日期 | 2024-03-30 |
| 专利号 | IN202421026424-A |
| 成果简介 | NOVELTY - Constructing and expressing a recombinant protective antigen (PA) involves: (a) gene cloning a protective antigen PA63 structural in a DNA plasmid; (b) using DNA chimeras bearing a set of signal sequences targets protective antigen PA63 to multiple subcellular locations, and expressing multiple forms of protease-cleaved PA63 fragment; (c) using an expressed recombinant protective antigen rPA63 protein to mediate and function as the receptor binding moiety of anthrax toxin complex for producing higher anti-protective antigen (PA) immunoglibulin (Ig)G1 antibody in a individual; (d) inducing a cellular and humoral immune response by expressed recombinant protective antigen rPA63 protein, where the DNA chimeras bearing a set of signal sequences includes lysosome-associated membrane protein 1 (LAMP1), tissue plasminogen activator (TPA) and ubiquitin. USE - Method for constructing and expressing a recombinant protective antigen (PA). ADVANTAGE - The recombinant protective antigen (PA) when administered produces multiple immune responses, where immune response include B and T-cell responses, Th-cell response, Cytotoxic T lymphocyte (CTL) response and α-(L)-threofuranosyl nucleic acid (TNA) response. The TNA titers to predict any correlation between the presence of TNA in the serum and protection against lethal challenge. The high end-point titers of IgG antibodies are maintained until 24 week by high avidity toxin neutralizing antibodies (TNA) and effective cellular adaptive immunity in the systemic compartment. DETAILED DESCRIPTION - Constructing and expressing a recombinant protective antigen (PA) involves: (a) gene cloning a protective antigen PA63 structural in a DNA plasmid; (b) using DNA chimeras bearing a set of signal sequences targets protective antigen PA63 to multiple subcellular locations, and expressing multiple forms of protease-cleaved PA63 fragment; (c) using an expressed recombinant protective antigen rPA63 protein to mediate and function as the receptor binding moiety of anthrax toxin complex for producing higher anti-protective antigen (PA) immunoglibulin (Ig)G1 antibody in a individual; (d) inducing a cellular and humoral immune response by expressed recombinant protective antigen rPA63 protein, where the DNA chimeras bearing a set of signal sequences includes lysosome-associated membrane protein 1 (LAMP1), tissue plasminogen activator (TPA) and ubiquitin, and the DNA plasmid include Protein Phosphatase 2 Phosphatase Activator-protective antigen-63 (pTPA-PA63), pPA63-Native, pLAMP1-PA63, pTPA-PA63-LAMP1, pUQ-PA63 and pTPA-P, and DNA plasmid encoding multiple forms of protease-cleaved PA63 fragment is expressed. |
| IPC 分类号 | C12N-001/21 ; C12N-015/70 ; C12N-015/80 ; C12P-021/02 |
| 国家 | 印度 |
| 专业领域 | 医药卫生 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/17444 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | 1.SUKINDER P S (SUKI-Individual) 2.HARSHIL K (HARS-Individual) 3.KHANSAHAB H M S (KHAN-Individual) 4.NILESHBHAI N (NILE-Individual) 5.CHODAVADIYA C (CHOD-Individual) 6.CHAUHAN D (CHAU-Individual) 7.PAREKH V (PARE-Individual) 8.SINGH C (SING-Individual) |
| 推荐引用方式 GB/T 7714 | SUKINDER P S,HARSHIL K,KHANSAHAB H M S,et al. Constructing and expressing a recombinant protective antigen (PA) involves gene cloning protective antigen PA63 structural in DNA plasmid, and using DNA chimeras bearing a set of signal sequences targets protective antigen PA63 to subcellular locations. IN202421026424-A[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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