METHOD FOR ASSESSING ACTIVITY OF MAMMALIAN NUCLEOTIDE EXCISION REPAIR SYSTEM
	POPOV A A; PETRUSEVA I O; SHAMANIN V A; LAVRIK O I
	2024-04-11
专利权人	AS SIBE CHEM BIOLOGY & FUNDAMENTAL MEDIC (ASIC-Non-standard)
申请日期	2024-04-11
专利号	RU2831226-C1
成果简介	NOVELTY - What is presented is a method for assessing the activity of a mammalian nucleotide excision repair (NER) system. Model DNA is obtained, which is a DNA duplex with size of 160 base pairs, both chains of which contain non-nucleoside inserts. Obtained model DNA is exposed to cell extracts containing NER proteins, which leads to removal of a volumetric damage in the composition of the adjacent DNA chain fragment. Chain integrity is restored by the presence of Taq-DNA polymerase and dNTP in the reaction mixture. Recovered DNA is amplified during subsequent PCR. USE - Biochemistry; molecular biology. ADVANTAGE - Disclosed method has high sensitivity of the method, and also enables to reduce time spent on measurement. 1 cl, 3 dwg, 1 tbl, 2 ex
IPC 分类号	C12Q-001/68
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/17235
专题	中国科学院新疆生态与地理研究所
作者单位	AS SIBE CHEM BIOLOGY & FUNDAMENTAL MEDIC (ASIC-Non-standard)
推荐引用方式 GB/T 7714	POPOV A A,PETRUSEVA I O,SHAMANIN V A,et al. METHOD FOR ASSESSING ACTIVITY OF MAMMALIAN NUCLEOTIDE EXCISION REPAIR SYSTEM. RU2831226-C1[P]. 2024.

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