METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs196336 (T greater than C) OF THE BAG3 GENE IN HUMAN BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES
	BUSHUEVA O YU; KOBZEVA K A
	2024-04-19
专利权人	UNIV KURSK STATE MEDICAL (UYKU-Non-standard)
申请日期	2024-04-19
专利号	RU2825989-C1
成果简介	NOVELTY - Invention relates to the field of biotechnology, molecular-genetic diagnostics and can be used in medicine in determining the hereditary predisposition to the development of diseases associated with the carriage of the polymorphic variant rs196336 (T>C) of the BAG3 gene. Disclosed is a method for genotyping polymorphic rs196336 (T>C) of the BAG3 gene in a human by real-time PCR using allele-specific fluorescent probes, which involves PCR using specially selected primers (forward 5'-CACCTGCAGTCTTCCTGGAT-3' and reverse 5'-CATCCGGCCTCTGTTTATTC-3') and probes with fluorophores (T-allele-specific fluorescent-labelled probe 5'-(FAM)CAGGCCCTTTCCTGGC(RTQ1)-3' and C-allele-specific fluorescent-labelled probe 5'-(ROX)CAGGCCCCTTCCTGGC(BHQ2)-3') in an amplifier with fluorescence detection. USE - Biotechnology. ADVANTAGE - Invention widens the range of methods for genotyping polymorphic variants of the BAG3 gene, is characterized by simplicity, accuracy and low cost. 1 cl, 1 dwg
IPC 分类号	C12N-015/00 ; C12Q-001/68
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/17100
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV KURSK STATE MEDICAL (UYKU-Non-standard)
推荐引用方式 GB/T 7714	BUSHUEVA O YU,KOBZEVA K A. METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs196336 (T greater than C) OF THE BAG3 GENE IN HUMAN BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES. RU2825989-C1[P]. 2024.

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