| METHOD OF IDENTIFYING GENES CODING AHL-LACTONASE (AIIA) AND LACCASE (COTA) IN BACTERIA OF GENUS BACILLUS | |
| 2024-04-22 | |
| 专利权人 | AGROHOLOD LLC (AGRO-Non-standard) |
| 申请日期 | 2024-04-22 |
| 专利号 | RU2834022-C1 |
| 成果简介 | NOVELTY - Disclosed is a method of identifying genes coding AHL-lactonase (aiiA) and laccase (cotA) in bacteria of the genus Bacillus. Method consists in extraction of nucleic acid by any method which enables to obtain DNA for a reaction mixture in an amount of not less than 100 ng, followed by a polymerase chain reaction using a set of original primers: cotA (Bacillus subtilis subsp. subtilis str. 168): forward TCGGCACAACTGAAATATGGTC and reverse GTCTTTCCAGCCCTTTTCACTT; cotA (Bacillus pumilus strain WH4): forward TCCTGCCTGTCGATCATACC and reverse TGCTTGCTGGTGATTAGGGT, aiiA (Bacillus cereus B4264): forward and reverse TGTGCAGCGAACGGAATATGTGAATAGCGACTGATGGCCT, aiiA (Bacillus thuringiensis Bt407): forward TGAGCCGGACGACCTTTTAT and reverse GATGGCCTGGAGAATGACCT. PCR results are assessed by electrophoretic separation of amplification products based on the presence of target 1,000 bp fragment. USE - Biotechnology. ADVANTAGE - Invention enables identification of genes coding AHL-lactonase (aiiA) and laccase (cotA) in bacteria of the genus Bacillus, isolated from a fodder additive, or obtained from pure bacterial cultures. 1 cl, 12 dwg, 3 tbl, 1 ex |
| IPC 分类号 | C12Q-001/68 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/17071 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | AGROHOLOD LLC (AGRO-Non-standard) |
| 推荐引用方式 GB/T 7714 | CHISTYAKOV V A,MAZANKO M S,BELIK T V,et al. METHOD OF IDENTIFYING GENES CODING AHL-LACTONASE (AIIA) AND LACCASE (COTA) IN BACTERIA OF GENUS BACILLUS. RU2834022-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论