| METHOD OF DETECTING RNA OF VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER USING REAL-TIME RT-PCR | |
| 2024-04-27 | |
| 专利权人 | UNIV URAL FEDERAL (UYUR-C) |
| 申请日期 | 2024-04-27 |
| 专利号 | RU2834908-C1 |
| 成果简介 | NOVELTY - Disclosed is a method of detecting RNA of the virus of Crimean–Congo haemorrhagic fever using real-time RT-PCR, which includes RNA extraction from biological samples followed by a two-stage reverse transcription reaction and a polymerase chain reaction for the target RNA fragments of the virus genome using a set of oligonucleotide primers and the corresponding fluorescent-labelled probe, complementary to the segment S of the gene, encoding the nucleocapsid protein of the virus of Crimean–Congo haemorrhagic fever, characterized in that the sequence used as a direct primer is – atc ggc act agt tga agc cac, as a reverse primer – gtg tag ctt tcg tcc caa gat tt, as a probe – cgc cca tat atg agt gtg cct ggg with fluorescent mark R6G by 5'-end and with non-fluorescent damper BHQ1 for 3'-end. USE - Biotechnology; medicine. ADVANTAGE - Invention makes it possible to monitor and diagnose the virus of Crimean–Congo haemorrhagic fever in healthy, sick and persons with suspected haemorrhagic fever caused by the virus, and epidemiological monitoring. 1 cl, 1 dwg, 3 tbl |
| IPC 分类号 | C12Q-001/68 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/16954 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV URAL FEDERAL (UYUR-C) |
| 推荐引用方式 GB/T 7714 | DOLGOVA A S,TURENKO A S,SHABALINA A V,et al. METHOD OF DETECTING RNA OF VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER USING REAL-TIME RT-PCR. RU2834908-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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