| METHOD OF PRODUCING INOCULUM WITH SINGLE NUMBER OF MICROORGANISMS | |
| 2024-05-06 | |
| 专利权人 | UNIV SAMARA MEDICAL (USMR-C) |
| 申请日期 | 2024-05-06 |
| 专利号 | RU2830890-C1 |
| 成果简介 | NOVELTY - Invention relates to microbiology and can be used to produce an inoculum with a single number of microorganisms. In contrast to existing methods, preparation of the working inoculum is carried out by performing a series of serial dilutions of the primary inoculum, which is prepared from the working culture using a nephelometer or according to laboratory turbidity standards at concentration of 1*108 CFU/ml. Primary inoculum is further diluted in series of 6 test tubes with 0.9% NaCl solution, where the number of CFU of the final test tube No. 6 will correspond to 1*102 CFU in 1 ml of suspension or 1*101 CFU in 0.1 ml of the suspension. Control of the number of CFU of the working inoculum is carried out by inoculation of its aliquot on 5 Petri dishes with Mueller-Hinton agar (MHA) for bacteria with common nutrient requirements and with addition of 5% mechanically defibrinated horse blood and 20 mg/l β-NAD for bacteria with complex nutritional needs (MHA-P) and subsequent counting of the number of CFU on Petri dishes, with the possibility of controlling the number of inoculum microorganisms with content thereof in range of 2-10 CFU per unit volume: in case there is no growth on plates with a nutrient medium or the number of CFU is less than 2, the series of dilutions is prepared again with a decrease in volume of 0.9% NaCl solution in all test tubes by 0.5 ml; if the number of CFU on the plates is more than 10, the series of dilutions is prepared repeatedly with increasing volume of 0.9% NaCl solution in all test tubes by 0.5 ml. USE - Microbiology. ADVANTAGE - Objective of the invention is to improve the method of producing inoculum with the possibility of controlling the number of microorganisms with content thereof in range of 2-10 CFU per unit volume. 1 cl |
| IPC 分类号 | C12Q-001/00 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/16871 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV SAMARA MEDICAL (USMR-C) |
| 推荐引用方式 GB/T 7714 | BOCHKAREVA P V,LYAMIN A V,ISMATULLIN D D,et al. METHOD OF PRODUCING INOCULUM WITH SINGLE NUMBER OF MICROORGANISMS. RU2830890-C1[P]. 2024. |
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