METHOD OF ISOLATING UNCULTURED FORMS OF STREPTOCOCCUS PNEUMONIAE
2024-05-16
专利权人UNIV SAMARA MEDICAL (USMR-C)
申请日期2024-05-16
专利号RU2828723-C1
成果简介NOVELTY - Disclosed is a method of isolating uncultivated forms of Streptococcus pneumoniae, characterized by the fact that strains of non-cultivated forms of Streptococcus pneumoniae are inoculated in a test tube with 5.0 ml of Mueller-Hinton broth with addition of 20% defibrinated mutton blood, the contents of the test tubes are mixed with a vortex shaker for 30 s, after which the obtained mixture is incubated at a temperature of +37 °C for 24 hours, as a result of which the obtained daily culture in test tubes is repeatedly mixed with a vortex shaker, 100 mcl of the content of the test tubes are taken for inoculation with a depleting stroke on 5% blood agar, then re-incubated at +37 °C and under conditions of 7% concentration of carbon dioxide until visible colonies appear. USE - Biotechnology. ADVANTAGE - Invention provides recovery of growth properties of non-cultivated forms of Streptococcus pneumonia and their recovery. 1 cl, 1 tbl
IPC 分类号C12N-001/02 ; C12N-001/20 ; C12Q-001/14 ; C12R-001/46
国家俄罗斯
专业领域生物科学
语种英语
成果类型专利
文献类型科技成果
条目标识符http://119.78.100.226:8889/handle/3KE4DYBR/16749
专题中国科学院新疆生态与地理研究所
作者单位
UNIV SAMARA MEDICAL (USMR-C)
推荐引用方式
GB/T 7714
ISMATULLIN D D,KAIUMOV K A,LYAMIN A V,et al. METHOD OF ISOLATING UNCULTURED FORMS OF STREPTOCOCCUS PNEUMONIAE. RU2828723-C1[P]. 2024.
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