| METHOD OF PRODUCING SECRETED STRESS PROTEINS OF FRANCISELLA TULARENSIS | |
| 2024-05-21 | |
| 专利权人 | MICROBE RUSSIAN ANTI-PLAGUE RES INST (MICR-Non-standard) |
| 申请日期 | 2024-05-21 |
| 专利号 | RU2830065-C1 |
| 成果简介 | NOVELTY - Invention relates to biotechnology and microbiology, and can be used for controlled synthesis of secreted stress proteins of Francisella tularensis. Disclosed is a method of producing secreted stress proteins of Francisella tularensis, involving stress exposure on a bacterial culture when cultivated in a liquid nutrient medium by adding 38% H2O2 to a final concentration of 5 mM and increasing temperature to 42 °C for 2 hours, sterilizing filtration of the culture liquid, precipitation of the protein fraction with ammonium sulphate at 55% to obtain Bfr proteins, at 70% to obtain GroEL/GroES proteins, followed by purification by gel chromatography and ion-exchange chromatography. USE - Biotechnology; microbiology. ADVANTAGE - Method enables increasing synthesis and secretion of stress proteins Bfr and GroEl/GroEs Francisella tularensis with subsequent recovery of these antigens in a biologically active state from the culture fluid without impurities of other components of the bacterial cell. 1 cl, 4 dwg, 2 tbl, 2 ex |
| IPC 分类号 | C12P-021/00 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/16688 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | MICROBE RUSSIAN ANTI-PLAGUE RES INST (MICR-Non-standard) |
| 推荐引用方式 GB/T 7714 | BORISOVA S V,VOLOKH O A,KIREEV M N,et al. METHOD OF PRODUCING SECRETED STRESS PROTEINS OF FRANCISELLA TULARENSIS. RU2830065-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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