| METHOD FOR DETECTING RNA OF MAMMARENAVIRUS GUANARITOENSE VIRUS BY RECOMBINASE POLYMERASE AMPLIFICATION | |
| 2024-05-28 | |
| 专利权人 | UNIV URAL FEDERAL (UYUR-C) |
| 申请日期 | 2024-05-28 |
| 专利号 | RU2834909-C1 |
| 成果简介 | NOVELTY - Disclosed is a method for detecting Mammarenavirus guanaritoense virus by isothermal recombinase polymerase amplification with real-time reverse transcription. Method involves extraction of RNA from biological samples, analysis using reverse transcriptase M-MuLV, specific primers RPA_GTOV_F5'-AAAAGCTTGAAGCCCTCTGTTAAGACCATCGG-3', RPA_GTOV_R5'-CGAAATTTGACAATGGATGAAAAAGTTGTAGTT-3', and fluorescent-labelled DNA probe Exo_GTOV5'-GCAATGCCTCCTTCTGATAAGACAGTTCAGG[FAM-dT]A[dSpacer]A[BHQ1-dT]CAGGTACTTGCCTTC[3'-C3-spacer]-3'. [dSpacer] modification used is the compound dSpacer CE phosphoramidite=5'-O-dimethoxytrityl-1',2'-dideoxyribose-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite. Results are interpreted based on the presence or absence of a difference in the fluorescence curve of the sample from the negative control sample which does not contain Mammarenavirus guanaritoense RNA. Result is considered to be positive if the value of the fluorescent signal increases with time and the final value by 30 minutes of analysis exceeds the value in the negative control sample. USE - Medical biotechnology. ADVANTAGE - Method makes it possible to quickly and reliably identify genetic material of Mammarenavirus guanaritoense virus in biological samples and other virus-containing samples. 1 cl, 1 dwg, 2 tbl |
| IPC 分类号 | C12Q-001/68 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/16550 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV URAL FEDERAL (UYUR-C) |
| 推荐引用方式 GB/T 7714 | KAPITONOVA M A,SHABALINA A V,DEDKOV V G,et al. METHOD FOR DETECTING RNA OF MAMMARENAVIRUS GUANARITOENSE VIRUS BY RECOMBINASE POLYMERASE AMPLIFICATION. RU2834909-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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