METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs862832 (CT) OF HSPA12B GENE IN HUMAN BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES
	BUSHUEVA O YU; KOBZEVA K A
	2024-06-03
专利权人	UNIV KURSK STATE MEDICAL (UYKU-Non-standard)
申请日期	2024-06-03
专利号	RU2828035-C1
成果简介	NOVELTY - Invention relates to biotechnology, molecular genetic diagnostics, and can be used in medicine in determining hereditary predisposition to developing diseases associated with carriage of polymorphic variant rs862832 (C>T) of the HSPA12B gene. Disclosed is a method for genotyping a polymorphic variant rs862832 (C>T) of the HSPA12B gene in a human by real-time PCR using allele-specific fluorescent probes, involving PCR using specially selected primers (forward 5′-TGTGGTGGTGGCAGCTAC-3′ and reverse 5′-CAGCATTTCAGGGCAGGAC-3′) and probes with fluorophores (C-allele-specific fluorescent-labelled probe 5′-(FAM)CAGGGCTACTCCACTCCCTCC(RTQ1)-3′ and T-allele-specific fluorescent-labelled probe 5′-(ROX)CAGGGCTACTCTACTCCCTCC(BHQ2)-3′) in an amplifier with fluorescence detection. USE - Biotechnology. ADVANTAGE - Invention widens the range of methods for genotyping polymorphic variants of the HSPA12B gene, is characterized by simplicity, accuracy and low cost. 1 cl, 1 dwg
IPC 分类号	C12Q-001/6886
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/16460
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV KURSK STATE MEDICAL (UYKU-Non-standard)
推荐引用方式 GB/T 7714	BUSHUEVA O YU,KOBZEVA K A. METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs862832 (CT) OF HSPA12B GENE IN HUMAN BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES. RU2828035-C1[P]. 2024.

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