METHOD FOR DETERMINING OXYGEN-INDEPENDENT BACTERICIDAL ACTIVITY OF NEUTROPHIL GRANULOCYTES IN SENSITIZATION WITH BRUCELLA
	MANAKOVA O O; YANCHENKO T A; VLASENKO V S
	2024-06-05
专利权人	SUPERHARD & NEW CARBON MATERIALS INST (SUPE-Non-standard)
申请日期	2024-06-05
专利号	RU2834930-C1
成果简介	NOVELTY - Disclosed is a method for determining oxygen-independent bactericidal activity of neutrophilic granulocytes in sensitization with brucellae, involving staging a reaction for determining the number of cationic proteins of neutrophils using a flat-bottomed plate for enzyme immunoassay, recording the reaction results by a photometric method at wavelength of 540 nm, determining a stimulation coefficient (SC), wherein the reaction activator used is an ultrasonic lysate of Brucella abortus 19 at concentration of 50 mcg/ml of protein in the end product; if the SC value is from 1.06 and more, the presence of specific sensitization of neutrophil granulocytes to brucella is determined. USE - Biotechnology; veterinary science. ADVANTAGE - Invention provides higher accuracy and reliability of assessing oxygen-independent bactericidal activity of neurophils in animals sensitized by brucella. 1 cl, 4 tbl
IPC 分类号	A61K-039/10 ; G01N-033/48 ; G01N-033/569
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/16405
专题	中国科学院新疆生态与地理研究所
作者单位	SUPERHARD & NEW CARBON MATERIALS INST (SUPE-Non-standard)
推荐引用方式 GB/T 7714	MANAKOVA O O,YANCHENKO T A,VLASENKO V S. METHOD FOR DETERMINING OXYGEN-INDEPENDENT BACTERICIDAL ACTIVITY OF NEUTROPHIL GRANULOCYTES IN SENSITIZATION WITH BRUCELLA. RU2834930-C1[P]. 2024.

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