| METHOD FOR GENOTYPING SINGLE-NUCLEOTIDE VARIANT rs2097461 (T greater than C) OF HUMAN XBP1 GENE BY REAL-TIME POLYMERASE CHAIN REACTION | |
| 2024-07-04 | |
| 专利权人 | UNIV KURSK MED (UYKU-Non-standard) |
| 申请日期 | 2024-07-04 |
| 专利号 | RU2831202-C1 |
| 成果简介 | NOVELTY - Invention relates to biotechnology, particularly to a method for genotyping a single nucleotide variant rs2097461 (T>C) of the human XBP1 gene. Said method is carried out by a real-time polymerase chain reaction and a forward primer XBP1-F, reverse primer XBP1, as well as XBP1-T- and C-allele-specific fluorescent-labelled probes are used, wherein for allele T homozygous DNA samples with genotype rs2097461-T/T fluorescence increase in FAM channel is detected, for allele C homozygous DNA samples with genotype rs2097461-C/C signal is detected by channel ROX, for heterozygous samples with genotype rs2097461-T/C there is increase of fluorescence by both channels of detection. USE - Biotechnology. ADVANTAGE - Present invention provides a simple, fast and cost-effective method of genotyping a single nucleotide variant rs2097461 (T>C) of the human XBP1 gene by real-time polymerase chain reaction. 1 cl, 2 dwg, 3 ex |
| IPC 分类号 | C12Q-001/68 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/15966 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV KURSK MED (UYKU-Non-standard) |
| 推荐引用方式 GB/T 7714 | AZAROVA YU E,POSTNIKOVA M I,KLESOVA E I,et al. METHOD FOR GENOTYPING SINGLE-NUCLEOTIDE VARIANT rs2097461 (T greater than C) OF HUMAN XBP1 GENE BY REAL-TIME POLYMERASE CHAIN REACTION. RU2831202-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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