| METHOD OF ISOLATING TOTAL DNA OF BACTERIA FROM SOIL SAMPLES, METHOD OF ASSESSING BACTERIAL COMPOSITION OF SOILS BY METAGENOMIC SEQUENCING AND KITS FOR IMPLEMENTING METHODS | |
| 2024-07-05 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2024-07-05 |
| 专利号 | RU2829656-C1 |
| 成果简介 | NOVELTY - Invention relates to biotechnology, particularly to a method of isolating total metagenomic DNA of bacteria from soil samples. Said method includes: collecting soil samples, simultaneous lysis of bacterial cells and homogenisation using a lysis solution, centrifuging said mixture followed by precipitation of bacterial DNA from the supernatant, successive washing of sorbent with DNA, drying of washed sorbent and DNA elution. Invention also relates to a kit for realizing said method, as well as a method for assessing the bacterial composition of soils and a kit of sets which enables such an assessment. USE - Biotechnology. ADVANTAGE - Present invention provides a highly efficient method of isolating metagenomic DNA of soil bacteria with a high degree of purification. 5 cl, 9 dwg, 7 tbl, 8 ex |
| IPC 分类号 | C12N-015/10 ; C12Q-001/68 ; C12Q-001/686 ; G01N-001/00 ; G01N-033/24 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/15943 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | IGNATEVA D I,VOLKOV A A,SHVARTSEV A A,et al. METHOD OF ISOLATING TOTAL DNA OF BACTERIA FROM SOIL SAMPLES, METHOD OF ASSESSING BACTERIAL COMPOSITION OF SOILS BY METAGENOMIC SEQUENCING AND KITS FOR IMPLEMENTING METHODS. RU2829656-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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