| METHOD FOR INDIRECT DETERMINATION OF TITRE OF INFECTIOUS ACTIVITY OF VACCINE STRAIN "LAVR" OF INFECTIOUS FELINE RHINOTRACHEITIS CAUSATIVE AGENT IN RAW MATERIAL FOR VACCINE BASED ON ANALYSIS OF MELTING CURVES OF HIGH-RESOLUTION AMPLICONS OF GENE UL47 USING DSSAFE FLUORESCENT DYE | |
| 2024-07-16 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2024-07-16 |
| 专利号 | RU2834233-C1 |
| 成果简介 | NOVELTY - Invention relates to biotechnology, specifically to a method for indirect determination of the infectious activity titre of the "Lavr" vaccine strain of the infectious feline rhinotracheitis agent in the vaccine raw material based on the analysis of melting curves of high-resolution amplicons of the UL47 gene using the dsSafe fluorescent dye. USE - Biotechnology. ADVANTAGE - Main advantages of the proposed invention are the possibility to reduce the time of analysis of virus-containing suspensions when determining the titre of infectious activity of the strain "Lavr" of the feline IRT agent in the raw material for vaccine to 2.5 hours; reduce the cost of one examination by 26.62%; increase analytical sensitivity to 0.1lgTCD50/cm3 due to use of highly specific primers and use of dsSafe fluorescent dye; increase the reliability of the analysis due to the establishment of the relationship between the titre of the infectious activity of the vaccine strain "Lavr" of the feline IRT agent in the raw material for the vaccine and the quantification cycle dsSafe presented in the form of a logarithmic function with high accuracy of approximation of 0.9987 and amplification efficiency of 99.85%. 2 cl, 2 dwg, 6 tbl, 5 ex |
| IPC 分类号 | C12Q-001/68 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/15780 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | DORONIN M I,MIKHALISHIN D V,GALKINA T S,et al. METHOD FOR INDIRECT DETERMINATION OF TITRE OF INFECTIOUS ACTIVITY OF VACCINE STRAIN "LAVR" OF INFECTIOUS FELINE RHINOTRACHEITIS CAUSATIVE AGENT IN RAW MATERIAL FOR VACCINE BASED ON ANALYSIS OF MELTING CURVES OF HIGH-RESOLUTION AMPLICONS OF GENE UL47 USING DSSAFE FLUORESCENT DYE. RU2834233-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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