| METHOD FOR ANALYSIS OF PDX MODEL OF COLORECTAL CANCER | |
| 2024-07-16 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2024-07-16 |
| 专利号 | RU2835140-C1 |
| 成果简介 | NOVELTY - Invention refers to medicine, namely to experimental oncology, and can be used to study a patient-derived xenograft (PDX) model of colorectal cancer. Tumour fragment of human colorectal cancer is sampled. Immunodeficient mice of Balb/c Nude line are anaesthetised, implantation of the above tumour fragment with size of 3 mm × 3 mm × 3 mm into an orthotopic site of anaesthetised mice is performed. Tumour node is excised when volume reaches 100 mm3, and is divided into 9 fragments: 1 fragment for histological examination, 4 fragments for molecular genetic analysis of KRAS, NRAS, BRAF gene mutation status and MSI status, 4 more fragments are placed in a cryomix tube, then the fragments are cryopreserved and stored in freezer for 90 days at a temperature of -80 °C. USE - Medicine. ADVANTAGE - Method enables examining the PDX model of colorectal cancer by determining the tumour histotype and KRAS, NRAS, BRAF gene mutation status and MSI status. 1 cl, 4 dwg, 1 tbl, 1 ex |
| IPC 分类号 | G09B-023/28 ; G16H-015/00 ; G16H-050/50 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/15778 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | GONCHAROVA A S,GALINA A V,MAKSIMOV A YU,et al. METHOD FOR ANALYSIS OF PDX MODEL OF COLORECTAL CANCER. RU2835140-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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