METHOD OF PRODUCING HETEROGENEOUS BIOCATALYST BASED ON CELLULASE IMMOBILIZED ON CATION-EXCHANGE FIBRE -1 IN -FORM
	DUBOVITSKAIA A N; KHOLYVKA M G; ARTYUKHOV V G
	2024-07-24
专利权人	FEDERAL STATE BUDGETARY EDUCATIONAL (BUDG-Non-standard)
申请日期	2024-07-24
专利号	RU2834720-C1
成果简介	NOVELTY - Disclosed is a method of producing a heterogeneous biocatalyst based on cellulase recovered from Aspergillus niger and immobilized on a cation-exchange fibre “ВИОН КН-1” in the “Н”-form, involving holding a cation exchanger in 0.1 M acetate buffer solution, separation of fibre from buffer by decantation and addition of 20 ml of cellulase solution in concentration of 10 mg/ml in 0.1 M acetate buffer with pH=4.5 to 1 g of cation-exchange fibre maintained in buffer, then incubated with stirring, the external solution is separated from the cellulase immobilized on the cation exchanger by centrifugation, followed by decantation of the external solution. Biocatalyst is washed twice by dialysis in a cellophane bag with a pore diameter of 25 kDa until there is no cellulase in the solution. USE - Biotechnology. ADVANTAGE - Invention provides higher specific activity of immobilized cellulase preparations. 1 cl, 4 dwg
IPC 分类号	C12N-011/02 ; C12N-009/42
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/15671
专题	中国科学院新疆生态与地理研究所
作者单位	FEDERAL STATE BUDGETARY EDUCATIONAL (BUDG-Non-standard)
推荐引用方式 GB/T 7714	DUBOVITSKAIA A N,KHOLYVKA M G,ARTYUKHOV V G. METHOD OF PRODUCING HETEROGENEOUS BIOCATALYST BASED ON CELLULASE IMMOBILIZED ON CATION-EXCHANGE FIBRE -1 IN -FORM. RU2834720-C1[P]. 2024.

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