| Culturing and expanding human dental pulp stem cells used in regenerative medicine, tissue engineering, and dental research, involves washing pulp tissue in phosphate buffer saline and chopping it into pieces in a media containing antibiotics, and treating with collagenase and dispase in water bath | |
| 2024-07-31 | |
| 专利权人 | UNIV KALINGA IND TECHNOLOGY INST (UYKA-Non-standard) |
| 申请日期 | 2024-07-31 |
| 专利号 | IN202431058179-A |
| 成果简介 | NOVELTY - Culturing and expanding human dental pulp stem cells (DPSCs) involves: (a) washing pulp tissue in 1x phosphate buffer saline (PBS) and chopping it into pieces in a media containing antibiotics; (b) treating with 0.1 wt.% collagenase and 50 U/ml dispase in a 37℃ water bath for 2 hours with continuous agitation; (c) incubating the cells for 1 hour at 37℃, then sieving through a 70 μm cell strainer, and centrifuging at speed of 1000 revolution/minute (rpm) for 5 minutes; (d) seeding onto 60 mm dishes in Dulbecco's Modified Eagle Medium (DMEM)/F12 containing 20 wt.% fetal bovine serum (FBS), 1.5mM L-glutamine, 2 wt.% antibiotic (100 U/ml penicillin and 10 mg/ml streptomycin), and growth factors; (e) allowing growth for 7 days, incubated at 37℃ with 5 vol.% carbon dioxide (CO2); and (f) changing the medium in every 3-4 days, and maintaining cells up to 3-4 passages within which all experiments are performed. USE - Method for culturing and expanding human dental pulp stem cells used in regenerative medicine, tissue engineering, and dental research. ADVANTAGE - The culture medium used in the method supports the proliferation and maintenance of DPSCs in an optimal environment. |
| IPC 分类号 | A61K-035/545 ; C12N-005/00 |
| 国家 | 印度 |
| 专业领域 | 医药卫生 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/15609 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV KALINGA IND TECHNOLOGY INST (UYKA-Non-standard) |
| 推荐引用方式 GB/T 7714 | DAS B,KUNDU C N,PATRI G,et al. Culturing and expanding human dental pulp stem cells used in regenerative medicine, tissue engineering, and dental research, involves washing pulp tissue in phosphate buffer saline and chopping it into pieces in a media containing antibiotics, and treating with collagenase and dispase in water bath. IN202431058179-A[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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