| METHOD FOR DIFFERENTIATION OF VACCINE STRAIN "ARCHIE" OF CAUSATIVE AGENT OF CANINE PARVOVIRUS ENTERITIS FROM OTHER STRAINS AND FIELD ISOLATES OF CANINE PVE USING HIGH-SENSITIVITY HRM-ANALYSIS USING TANDEM FLUORESCENT DYE SN v428 | |
| 2024-07-31 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2024-07-31 |
| 专利号 | RU2835907-C1 |
| 成果简介 | NOVELTY - Invention relates to veterinary virology, to means of molecular diagnostics, in particular, to differentiation of the vaccine strain "Archie" of the causative agent of canine parvoviral enteritis from other strains and field isolates of PVE using a highly sensitive HRM-analysis using a tandem fluorescent dye SN v428. Main advantage of the present invention is the possibility of performing differential analysis in a short period of time – not more than 2 hours. Developed method was used for the first time to solve this problem. In the target DNA region of the VP1 gene of the causative agent of parvoviral enteritis, there are two nucleotide mutations unique to the vaccine strain "Archie", which made it possible to develop highly specific original oligonucleotide primers CPV-F2524 and CPV-R2676, calculated for amplification of the target DNA region of the VP1 gene of the causative agent of parvoviral enteritis of size 176 bp and analysis of maximum melting points of PCR products using tandem fluorescent dye SN v428. USE - Virology; veterinary science. ADVANTAGE - Developed method is characterized by 100% analytical specificity; in 95% confidence interval diagnostic sensitivity for this method is 99.05-100%, diagnostic specificity – 98.62-100%. 2 cl, 4 dwg, 6 tbl, 3 ex |
| IPC 分类号 | C12Q-001/68 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/15606 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | DORONIN M I,MIKHALISHIN D V,GALKINA T S,et al. METHOD FOR DIFFERENTIATION OF VACCINE STRAIN "ARCHIE" OF CAUSATIVE AGENT OF CANINE PARVOVIRUS ENTERITIS FROM OTHER STRAINS AND FIELD ISOLATES OF CANINE PVE USING HIGH-SENSITIVITY HRM-ANALYSIS USING TANDEM FLUORESCENT DYE SN v428. RU2835907-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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