METHOD FOR PREPARING AN ALLOGENIC AMNIOTIC MEMBRANE FOR RECONSTRUCTIVE MEDICINE
	VOLOVA L T; MILYUDIN E S; KUCHUK K E
	2024-08-14
专利权人	UNIV SAMARA MEDICAL (USMR-C)
申请日期	2024-08-14
专利号	RU2835347-C1
成果简介	NOVELTY - Invention relates to medicine, to biotechnology, in particular to methods for making bioimplants from a connective tissue, and can be used in preparing an allogenic amniotic membrane for reconstructive medicine. Amniotic membrane is mechanically separated from the chorion and washed in running water from blood clots for at least 30 minutes. Then it is placed into an ultrasonic chamber filled with degassed distilled water with a temperature of +23 °C, and for at least 3 minutes are treated with ultrasound with frequency of 20...45 kHz and intensity of 3 W/cm2, after that, it is placed for 15 minutes in a chamber in which a vacuum is created with a residual pressure of 1…2 Pa. Washing is carried out in degassed distilled water, after extraction from the chamber, the biomaterial is washed in solution of 0.9 % NaCl, frozen at a temperature of -20...-60 °C, lyophilized, sealed and sterilized by radiation. USE - Biotechnology. ADVANTAGE - Invention enables to produce a biomaterial from an amniotic membrane with preservation of elasticity and decellularisation. 1 cl, 2 dwg, 2 ex
IPC 分类号	A61K-035/50 ; A61L-027/38 ; C12N-005/073 ; G01N-033/48
国家	俄罗斯
专业领域	化学化工
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/15450
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV SAMARA MEDICAL (USMR-C)
推荐引用方式 GB/T 7714	VOLOVA L T,MILYUDIN E S,KUCHUK K E. METHOD FOR PREPARING AN ALLOGENIC AMNIOTIC MEMBRANE FOR RECONSTRUCTIVE MEDICINE. RU2835347-C1[P]. 2024.

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