TEST SYSTEM FOR DETECTION OF APPLE CHLOROTIC LEAFSPOT VIRUS BY REAL-TIME PCR METHOD
2024-08-21
专利权人FEDERAL STATE BUDGETARY INST (FSBI-C)
申请日期2024-08-21
专利号RU2835209-C1
成果简介NOVELTY - Described is a test system for detecting chlorotic leafspot virus of apple leaves, comprising a mixture for carrying out amplification, consisting of a reaction buffer, deoxynucleotide triphosphates, two oligonucleotide primers Seq1 and Seq2, specific to a fragment of a gene encoding a virus envelope protein, one oligonucleotide probe Seq3 labelled with a fluorescent dye FAM. Mixture also includes a positive control sample, which is a recombinant plasmid DNA Seq4 containing the corresponding fragment of the virus genome. USE - Molecular biology. ADVANTAGE - Invention enables highly specific identification of DNA of chlorotic leafspot virus of apple-tree leaves, with high sensitivity. 1 cl, 4 dwg, 3 tbl
IPC 分类号C12Q-001/68 ; C12Q-001/6876
国家俄罗斯
专业领域生物科学
语种英语
成果类型专利
文献类型科技成果
条目标识符http://119.78.100.226:8889/handle/3KE4DYBR/15392
专题中国科学院新疆生态与地理研究所
作者单位
FEDERAL STATE BUDGETARY INST (FSBI-C)
推荐引用方式
GB/T 7714
STAKHEEV A A,RYAZANTSEV D YU,SAMOKHVALOVA L V,et al. TEST SYSTEM FOR DETECTION OF APPLE CHLOROTIC LEAFSPOT VIRUS BY REAL-TIME PCR METHOD. RU2835209-C1[P]. 2024.
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