METHOD FOR OBTAINING ASEPTIC CULTURE IN VITRO OF HARDWOOD PLANTS OF MIDDLE AND SENIOR CLASSES OF AGE
2024-08-28
专利权人UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C)
申请日期2024-08-28
专利号RU2834363-C1
成果简介NOVELTY - Invention relates to biotechnology and can be used for propagation of hardwood plants of middle and senior age classes in conditions of tissue culture. Method of clonal micropropagation involves harvesting branches from mother woody plants of middle and senior age classes, forcing etiolated shoots, selection of primary explants, their sterilization, cultivation on a nutrient medium based on MS macro- and microsalts, sterilization of primary explants in 1% working solution of a disinfectant based on sodium hypochlorite (with active chlorine content of 5-15%) and 5% solution of Lysoformin 3000 and culturing explants on a sterile nutrient medium for 15-20 days. USE - Biotechnology. ADVANTAGE - Method for in vitro introduction of hardwood plants of middle and senior age classes enables to obtain regenerated plants for their subsequent reproduction in order to preserve the gene pool of the initial parent forms and expand the collection fund. 1 cl
IPC 分类号A01H-004/00
国家俄罗斯
专业领域生物科学
语种英语
成果类型专利
文献类型科技成果
条目标识符http://119.78.100.226:8889/handle/3KE4DYBR/15338
专题中国科学院新疆生态与地理研究所
作者单位
UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C)
推荐引用方式
GB/T 7714
ALEKSANDROVA I V,LEBEDEVA O P,MELEKHOV V I,et al. METHOD FOR OBTAINING ASEPTIC CULTURE IN VITRO OF HARDWOOD PLANTS OF MIDDLE AND SENIOR CLASSES OF AGE. RU2834363-C1[P]. 2024.
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