| METHOD FOR GENOTYPING A SINGLE-NUCLEOTIDE VARIANT RS3219493 (C greater than G) OF THE HUMAN MUTYH GENE BY REAL-TIME POLYMERASE CHAIN REACTION | |
| 2024-09-05 | |
| 专利权人 | UNIV KURSK STATE MEDICAL (UYKU-Non-standard) |
| 申请日期 | 2024-09-05 |
| 专利号 | RU2835214-C1 |
| 成果简介 | NOVELTY - Described is a method for genotyping a single nucleotide variant of rs3219493 (C>G) human MUTYH gene by real-time polymerase chain reaction, characterized by that selected primers and fluorescent-labelled probes are used. For allele C homozygous DNA samples with genotype rs3219493-C/C, a fluorescence increase in FAM channel is detected, for DNA samples homozygous for allele G with genotype rs3219493-G/G the signal is detected in ROX channel. For heterozygous samples with genotype rs3219493-C/G, there is an increase in fluorescence in both detection channels, and the presence of two dyes FAM and ROX enables to determine the presence of each of the analysed alleles of the MUTYH gene in the analysed DNA sample and, accordingly, the human genotype. USE - Molecular biology. ADVANTAGE - Developing a simple and fast method for genotyping a single nucleotide variant of rs3219493 (C>G) human MUTYH gene by real-time polymerase chain reaction. 1 cl, 2 dwg, 3 ex |
| IPC 分类号 | C12Q-001/68 ; C12Q-001/6876 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/15253 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV KURSK STATE MEDICAL (UYKU-Non-standard) |
| 推荐引用方式 GB/T 7714 | AZAROVA YU E,SEMIKINA E V,KLESOVA E I,et al. METHOD FOR GENOTYPING A SINGLE-NUCLEOTIDE VARIANT RS3219493 (C greater than G) OF THE HUMAN MUTYH GENE BY REAL-TIME POLYMERASE CHAIN REACTION. RU2835214-C1[P]. 2024. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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