Point-of-care blood plasma-based biomarker detection system used for protein biomarkers detection in in-situ separated plasma, comprises a microfluidic strip comprising a surface-modified Polymethyl Methacrylate microfluidic strip including a separation portion including plasma separator portion
	CHAKRABORTY S; DAS S; MAIKAP A; ROY B
	2024-09-12
专利权人	INDIAN INST TECHNOLOGY KHARAGPUR (IIKH-C)
申请日期	2024-09-12
专利号	IN202431069154-A
成果简介	NOVELTY - Point-of-care blood plasma-based biomarker detection system comprises: (i) a microfluidic strip comprising a surface-modified Polymethyl Methacrylate (PMMA) microfluidic strip including a separation portion including plasma separator portion having Lateral Flow Assay (LFA) based in-situ plasma separator and a sensing portion including Carbon Dot (CD)-tagged secondary antibody probes and surface-immobilized primary antibodies, adapted for forming complexes upon sensing of plasma-based target biomarker in analytes; and (ii) an image acquisition unit comprising a fluorescent image acquisition unit for capturing fluorescent images of tagged analytes and intensity-based determinants of biomarker detection. USE - Point-of-care blood plasma-based biomarker detection system used for protein biomarkers detection in in-situ separated plasma. ADVANTAGE - The point-of-care blood plasma-based biomarker detection system detects protein biomarkers in in-situ separated plasma with sensitivity of limit of detection (LOD) of 0.4 ng/mL and 0.9 ng/ml and high specificity. The system has portable image acquisition unit and an AI framework to enable simultaneous analysis of multiple analytes with low-cost, and resource-efficient methods. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for manufacturing the point-of-care blood plasma-based biomarker detection system, which involves: (1) providing a microfluidic strip having a surface-modified Polymethyl Methacrylate (PMMA) microfluidic strip including a separation portion including a plasma separator part having Lateral Flow Assay (LFA) based in-situ plasma separator and a sensing portion provided with CD-tagged secondary antibody probes adapted for forming conjugates upon sensing of plasma-based target biomarker in analytes and binding with the primary antibodies forming a sandwich; and (2) providing an image acquisition unit comprising a fluorescent image acquisition unit for capturing fluorescent images of tagged analytes and intensity-based determinants of biomarker detection.
IPC 分类号	B01L-003/00 ; G01N-021/64 ; G01N-033/49 ; G01N-033/533 ; G01N-033/538 ; G01N-033/543 ; G01N-033/558 ; G01N-033/58
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/15183
专题	中国科学院新疆生态与地理研究所
作者单位	INDIAN INST TECHNOLOGY KHARAGPUR (IIKH-C)
推荐引用方式 GB/T 7714	CHAKRABORTY S,DAS S,MAIKAP A,et al. Point-of-care blood plasma-based biomarker detection system used for protein biomarkers detection in in-situ separated plasma, comprises a microfluidic strip comprising a surface-modified Polymethyl Methacrylate microfluidic strip including a separation portion including plasma separator portion. IN202431069154-A[P]. 2024.

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