Isolating DNA from mosquitoes involves taking 4 adult mosquitoes or 3rd instar larvae and placing them into a 1.5 ml microcentrifuge tube, homogenizing the sample completely using a micropestle, and adding 10X Tris-Phosphate ethylenediaminetetraacetic acid buffer to the homogenized sample
	DAS B
	2024-11-14
专利权人	UNIV KALINGA IND TECHNOLOGY INST (UYKA-Non-standard)
申请日期	2024-11-14
专利号	IN202431087857-A
成果简介	NOVELTY - Isolating DNA from mosquitoes involves: (a) taking 4 adult mosquitoes or 3rd instar larvae and placing them into a 1.5 ml microcentrifuge tube (MCT); (b) homogenizing the sample completely using a micropestle; (c) adding 40 μl 10X Tris-Phosphate ethylenediaminetetraacetic acid (TPE) buffer to the homogenized sample and vortexing the solution for 5 seconds; (d) incubating the solution at 70℃ and stirring speed of 900 revolution/minute (rpm) for 25 minutes using a thermomixer; (e) adding 100 mM sodium hydroxide (NaOH) buffer to the solution and mixing gently by inverting; (f) incubating the solution for 5 minutes at room temperature to facilitate cell lysis and DNA extraction; (g) allowing the cell debris to settle at the bottom of the MCT and collecting the supernatant containing the extracted DNA; and (h) transferring the supernatant to a fresh 1.5 ml microcentrifuge tube (MCT) and storing DNA in nanopure (NF) water at 4℃ for downstream PCR analysis and sequencing. USE - Method for isolating DNA from mosquitoes. ADVANTAGE - The method enables isolation of any mosquito DNA, provide a versatile solution for various mosquito species across diverse environments. The DNA isolation assay is field stable, scalable, and efficient, and provide a practical solution for DNA extraction in resource-limited and rural settings. The method not only simplifies the process of DNA extraction but also ensures high-quality results that are suitable for PCR amplification and species identification, and contribute significantly to the ongoing efforts in mosquito monitoring, vector control, and disease prevention.
IPC 分类号	C07H-021/04 ; C12N-015/10 ; C12P-019/34 ; C12Q-001/68
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/14542
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV KALINGA IND TECHNOLOGY INST (UYKA-Non-standard)
推荐引用方式 GB/T 7714	DAS B. Isolating DNA from mosquitoes involves taking 4 adult mosquitoes or 3rd instar larvae and placing them into a 1.5 ml microcentrifuge tube, homogenizing the sample completely using a micropestle, and adding 10X Tris-Phosphate ethylenediaminetetraacetic acid buffer to the homogenized sample. IN202431087857-A[P]. 2024.

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