Carrying out electrochemical detection of dengue NS1 protein in human serum samples by utilizing three-electrode system with a screen-printed carbon electrode as working electrode, and silver chloride as reference electrode, which involves modifying surface of working screen-printed carbon electrode
	LOKHANDE D C; KAPSE A N; PANDEYTIWARI A
	2024-11-25
专利权人	UNIV PATIL EDUCATION SOC D Y (UYPA-Non-standard)
申请日期	2024-11-25
专利号	IN202421091761-A
成果简介	NOVELTY - Carrying out electrochemical detection of dengue NS1 protein in human serum samples by utilizing a three-electrode system comprising a screen-printed carbon electrode (SPCE) as the working electrode, silver/silver chloride (Ag/AgCl) reference electrode, and carbon counter electrode, which involves: (a) modifying the surface of working screen-printed carbon electrode (SPCE) by applying an equimolar mixture of 0.1-2 M l-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and 0.1-2 M N-hydroxy succinimide (NHS). USE - Method for carrying out electrochemical detection of dengue NS1 protein in human serum samples by utilizing three-electrode system. ADVANTAGE - The method demonstrates high specificity and sensitivity, with no interference from other bioanalytes, making it a reliable and rapid diagnostic tool for detecting dengue infections. DETAILED DESCRIPTION - Carrying out electrochemical detection of dengue NS1 protein in human serum samples by utilizing a three-electrode system comprising a screen-printed carbon electrode (SPCE) as the working electrode, silver/silver chloride (Ag/AgCl) reference electrode, and carbon counter electrode, which involves: (a) modifying the surface of working screen-printed carbon electrode (SPCE) by applying an equimolar mixture of 0.1-2 M l-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and 0.1-2 M N-hydroxy succinimide (NHS); (b) adding 1-5 pL 0.1-10 pg/mL anti-dengue NS1 protein onto the working electrode surface and incubating for 5-40 minutes; (c) washing the working electrode with phosphate-buffered saline (PBS) and air-drying; (d) blocking non-specific binding sites on working electrode by applying 0.05-0.1 wt.% bovine serum albumin (BSA) solution followed by a 0.5-1 wt.% polyvinyl alcohol (PVA) coating and air-diying; and (e) recording peak current density by using differential pulse voltammetry (DPV) for detection of dengue NS1 protein.
IPC 分类号	C07K-014/005 ; C07K-016/10 ; G01N-033/569 ; G01N-033/574 ; H04L-005/00
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/14388
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV PATIL EDUCATION SOC D Y (UYPA-Non-standard)
推荐引用方式 GB/T 7714	LOKHANDE D C,KAPSE A N,PANDEYTIWARI A. Carrying out electrochemical detection of dengue NS1 protein in human serum samples by utilizing three-electrode system with a screen-printed carbon electrode as working electrode, and silver chloride as reference electrode, which involves modifying surface of working screen-printed carbon electrode. IN202421091761-A[P]. 2024.

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