Electrospun resistant starch as scaffold for delivery of probiotics, comprises resistant starch extracted from lotus stems (Nelumbo nucifera), polyvinyl-alcohol, surfactant and probiotic culture of bacteria Lactobacillus plantarum
	SHAH A; ASHWAR B A; BHAT N A; NOOR N; GANI A
	2024-11-27
专利权人	UNIV KASHMIR (UYKA-Non-standard)
申请日期	2024-11-27
专利号	IN202411092724-A
成果简介	NOVELTY - Electrospun resistant starch as scaffold comprises resistant starch (RS) extracted from lotus stems (Nelumbo nucifera), polyvinyl-alcohol (PVA), surfactant and probiotic culture of bacteria Lactobacillus plantarum. USE - Electrospun resistant starch as scaffold for delivery of probiotics. ADVANTAGE - The cell viability of Lactobacillus plantarum in the electrospun scaffold is in the range of 67.88 ± 0.12 to 82.55 ± 0.11% after electrospinning, survival rate of Lactobacillus planatrum cells in the electrospun scaffold during different time-temperature conditions is 2-fold to 3-fold as compared to free bacterial cells. The viability of Lactobacillus planatrum cells in the electrospun scaffold in simulated gastric juice is 6-8 fold as compared to free cells for 30 minutes to 60 minutes. The Lactobacillus planatrum cells show 4-6 fold alpha-glucosidase inhibition as compared to free cells. The electrospun fibers are loaded with probiotic cells, which survive high temperature and used in various baking and high heat processes, further RS-PVA is used as an important biopolymer for encapsulation of probiotics with increased viability and used for development of foods with enhanced bioactivity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method of preparing an electrospun resistant starch as a scaffold for delivery of probiotics, which involves: (1) extracting RS from lotus stems (Nelumbo nucifera), (2) activating probiotic culture of Lactobacillus plantarum (MTCC 021) bacteria in sterile De Man-Rogosa-Sharpe agar (MRS) broth media, by incubating at for 24 hours at 37℃ and harvesting the activated cells, (3) obtaining bacteria loaded RS solutions by adding RS in a concentration of 2 wt.%, 5 wt.% and 10 wt.% in sterile MilliQ water and keeping on a stirrer at speed of 400 revolution/minute (rpm) at a temperature of 30℃ for overnight yielding slurries, (4) adding 1 mL probiotic cultures in the slurries followed by the addition of 0.02mL/100 mL of surfactant to obtain probiotic-RS mixture; (5) agitating the probiotic-RS mixture vigorously using homogenizer to yield creamy emulsion, (6) dding 25 mL 1 wt.% PVA (polyvinyl alcohol) to the emulsion followed by homogenization for 2 minutes at 150 rpm to obtain homogenized probiotic RS-PVA solution; and (7) electrospinning the homogenized probiotic-RS-PVA solution at specific electrospinning parameters to obtain scaffold of desired characteristics.
IPC 分类号	A61K-031/715 ; A61K-035/744 ; A61K-036/62 ; C08L-005/00 ; C12N-001/20 ; C12N-011/04 ; C12P-019/04 ; D01D-005/00
国家	印度
专业领域	材料科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/14350
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV KASHMIR (UYKA-Non-standard)
推荐引用方式 GB/T 7714	SHAH A,ASHWAR B A,BHAT N A,et al. Electrospun resistant starch as scaffold for delivery of probiotics, comprises resistant starch extracted from lotus stems (Nelumbo nucifera), polyvinyl-alcohol, surfactant and probiotic culture of bacteria Lactobacillus plantarum. IN202411092724-A[P]. 2024.

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