Immobilizing lignocellulolytic enzymes on silica-doped biochar nanoparticles, involves obtaining biochar by pyrolyzing lignocellulosic biomass under controlled temperature and pressure, and treating biochar with acidic solution to remove alkali and alkaline earth metals and bio-oil residues
	VERMA A; PANDEY A K
	2024-12-15
专利权人	UNIV CHHATRAPATI SHAHU JI MAHARAJ (UYCH-Non-standard)
申请日期	2024-12-15
专利号	IN202411099235-A
成果简介	NOVELTY - Immobilizing lignocellulolytic enzymes on silica-doped biochar nanoparticles, involves: (a) obtaining biochar by pyrolyzing lignocellulosic biomass under controlled temperature and pressure conditions; (b) treating the biochar with an acidic solution to remove alkali and alkaline earth metals and bio-oil residues; (c) incorporating nano-sized silica into the biochar by ball-milling the biochar with silica particles; (d) dispersing the silica-doped biochar in a calcium chloride solution and subjecting the mixture to sonication and stirring; (e) drying the resultant solid mixture at a controlled temperature to obtain a homogenized composite; (f) performing pyrolysis of such composite in a tubular reactor under an inert atmosphere to form a silica-doped biochar nanoparticle material. USE - Method for immobilizing lignocellulolytic enzymes on silica-doped biochar nanoparticles. ADVANTAGE - The lignocellulolytic enzymes retains its activity and stability on the biochar surface, which contributes to efficient biomass saccharification by maintaining enzyme proximity to the lignocellulosic substrate during the saccharification process. The crosslinking process enables the composite to withstand operational stress during biomass processing, leading to consistent enzyme performance and high saccharification efficiency. DETAILED DESCRIPTION - Immobilizing lignocellulolytic enzymes on silica-doped biochar nanoparticles, involves: (a) obtaining biochar by pyrolyzing lignocellulosic biomass under controlled temperature and pressure conditions; (b) treating the biochar with an acidic solution to remove alkali and alkaline earth metals and bio-oil residues; (c) incorporating nano-sized silica into the biochar by ball-milling the biochar with silica particles; (d) dispersing the silica-doped biochar in a calcium chloride solution and subjecting the mixture to sonication and stirring; (e) drying the resultant solid mixture at a controlled temperature to obtain a homogenized composite; (f) performing pyrolysis of such composite in a tubular reactor under an inert atmosphere to form a silica-doped biochar nanoparticle material; (g) conducting surface functionalization of the silica-doped biochar nanoparticle material with functional groups selected from carboxyl, hydroxyl, or amine groups; (h) mixing a solution of activated lignocellulolytic enzyme with the functionalized silica-doped biochar nanoparticle material in the presence of a crosslinking agent; (i) rinsing the enzyme-immobilized biochar to remove unbound enzyme; and (j) drying the enzyme-immobilized biochar.
IPC 分类号	B01J-020/28 ; B82Y-030/00 ; C10B-053/02 ; C12N-009/42 ; C12P-019/02
国家	印度
专业领域	能源矿产
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/14062
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV CHHATRAPATI SHAHU JI MAHARAJ (UYCH-Non-standard)
推荐引用方式 GB/T 7714	VERMA A,PANDEY A K. Immobilizing lignocellulolytic enzymes on silica-doped biochar nanoparticles, involves obtaining biochar by pyrolyzing lignocellulosic biomass under controlled temperature and pressure, and treating biochar with acidic solution to remove alkali and alkaline earth metals and bio-oil residues. IN202411099235-A[P]. 2024.

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