New nucleotide sequence set comprising a forward primer and reverse primer, used in DNA-based identification kit for molecular identification of species of air-breathing catfish, where species of air-breathing catfish are Clarias magur and Clarias gariepinus
	SARKAR U K; KUSHWAHA B; NARAYAN D; DASMANDAL T; SINGH J; SINGH P J; GAUTAM R K; SAHU A; KUMAR S; SINGH M
	2024-12-16
专利权人	INDIAN COUNCIL AGRIC RES (ICOU-C)
申请日期	2024-12-16
专利号	IN202411099622-A
成果简介	NOVELTY - Nucleotide sequence set is new, where nucleotide sequence set comprises a forward primer and reverse primer having 20 nucleotides (SEQ ID NOs: 1-2), given in the specification. USE - Nucleotide sequence set used in DNA-based identification kit for molecular identification of species of air-breathing catfish, where species of air-breathing catfish are Clarias magur and Clarias gariepinus (claimed). ADVANTAGE - The nucleotide sequence set facilitates rapid species identification, completed within 24 hours, which significantly reduce the time required for regulatory and enforcement actions, ensure high accuracy in species differentiation and reduce the risk of false positives or negatives. The set is preferably adaptable for large-scale testing scenarios, making it suitable for conservation efforts, legal enforcement, and commercial applications. The method is configured to provide identification or differentiation of the species of air-breathing catfish at juvenile stages including fry, fingerling, and larval stages, and identification of processes samples including fish fillets and cooked fish. DETAILED DESCRIPTION - Nucleotide sequence set is new, where nucleotide sequence set comprises a forward primer and reverse primer having 20 nucleotides caaccacggtgttcttgcag (SEQ ID NO: 1), and ctcgcccgagcagtagttag (SEQ ID NO: 2). INDEPENDENT CLAIMS are included for: DNA-based identification kit, which comprises a nucleotide sequence set, reagents for polymerase chain reaction (PCR) amplification including DNA polymerase, deoxynucleotide triphosphates (dNTPs), and buffer, and instructions for performing a PCR-based identification process; and Method for identifying species of air-breathing catfish, which involves: (1) extracting genomic DNA from a sample; (2) amplifying a specific genomic DNA region shared by Clarias magur and Clarias gariepinus using a nucleotide sequence set to obtain an amplified product; and (3) identifying and differentiating Clarias magur and Clarias gariepinus by examining the amplified product.
IPC 分类号	C12N-015/11 ; C12N-015/63 ; C12Q-001/68 ; C12Q-001/6888
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/14032
专题	中国科学院新疆生态与地理研究所
作者单位	INDIAN COUNCIL AGRIC RES (ICOU-C)
推荐引用方式 GB/T 7714	SARKAR U K,KUSHWAHA B,NARAYAN D,et al. New nucleotide sequence set comprising a forward primer and reverse primer, used in DNA-based identification kit for molecular identification of species of air-breathing catfish, where species of air-breathing catfish are Clarias magur and Clarias gariepinus. IN202411099622-A[P]. 2024.

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