Forming non-destructive interface between bioanalyte and plasmonic metal nanostructure used for bio-sensing, involves mixing ethanol, methanol, deionized water followed by adding Nafion solution, then functionalized graphene, drop-casting the third sonicated mixuter on plasmonic metal nanostructure
	TAMANG J S; GUPTA D V V; PRANEL S V; MALIK N; VENUGOPALAN D; BOHL S S; ALAGESAN V; MAZUMDER N; SRINIVASULU M; PRASAD B A; MOORTHY V H S; MALLIKARJUN B
	2024-12-21
专利权人	MANIPAL ACAD HIGHER EDUCATION (MANI-Non-standard)
申请日期	2024-12-21
专利号	IN202441101653-A
成果简介	NOVELTY - Forming a non-destructive interface between a bioanalyte and plasmonic metal nanostructure, involves: (a) mixing 1ml ethanol, 1ml methanol, 1 ml de-ionized water in a vial and sonicating for 5 minutes to form a first sonicated mixture; (b) adding 0.1-5 ml of 5-50% Nafion solution to first the sonicated mixer and sonicating again for 20 minutes to form a second sonicated mixture; (c) adding 5-50 mg functionalized graphene to the second sonicated mixer and sonicating again for 20 minutes to form a third sonicated mixture; (d) drop-casting 10-100 µL prepared third sonicated mixer on a plasmonic metal nanostructure; (e) drying the cast at 28-35℃, humidity 40-65% chamber for 30 minutes to form a layer; and (f) performing successive iterations of drop-casting and controlled drying to achieve a plurality of layers of functionalized graphene on the plasmonic metal nanostructure, optimized to meet desired thickness and bio-analyte detection requirements. USE - Method for forming a non-destructive interface between a bioanalyte and plasmonic metal nanostructure for used in healthcare, environmental monitoring, and biotechnology, and for bio-sensing. ADVANTAGE - The method enables to enhance stability, improve plasmonic effects, and provide a cost-effective solution for large-scale biosensor; and achieve high sensitivity and specificity in detection. DETAILED DESCRIPTION - Forming a non-destructive interface between a bioanalyte and plasmonic metal nanostructure, involves: (a) mixing 1ml ethanol, 1ml methanol, 1 ml de-ionized water in a vial and sonicating for 5 minutes to form a first sonicated mixture; (b) adding 0.1-5 ml of 5-50% Nafion solution to first the sonicated mixer and sonicating again for 20 minutes to form a second sonicated mixture; (c) adding 5-50 mg functionalized graphene to the second sonicated mixer and sonicating again for 20 minutes to form a third sonicated mixture; (d) drop-casting 10-100 µL prepared third sonicated mixer on a plasmonic metal nanostructure; (e) drying the cast at a controlled temperature of 28-35℃, humidity 40-65% chamber for 30 minutes to form a layer; and (f) performing successive iterations of drop-casting and controlled drying to achieve a plurality of layers of functionalized graphene on the plasmonic metal nanostructure, optimized to meet desired thickness and bio-analyte detection requirements; where 0.5-10 µg/cm2functionalized graphene coating enhances Localized Surface Plasmon Resonance (LSPR) sensitivity by 10-20%, ensures non-destructive bio-analyte detection, and improves structural stability for advanced biosensing applications.
IPC 分类号	B82Y-005/00 ; C08K-003/04 ; G01N-033/50 ; G01N-033/543 ; H01L-031/0224
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/13893
专题	中国科学院新疆生态与地理研究所
作者单位	MANIPAL ACAD HIGHER EDUCATION (MANI-Non-standard)
推荐引用方式 GB/T 7714	TAMANG J S,GUPTA D V V,PRANEL S V,et al. Forming non-destructive interface between bioanalyte and plasmonic metal nanostructure used for bio-sensing, involves mixing ethanol, methanol, deionized water followed by adding Nafion solution, then functionalized graphene, drop-casting the third sonicated mixuter on plasmonic metal nanostructure. IN202441101653-A[P]. 2024.

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