Performing cotton thread chromatography, comprises taking cotton thread, spotting sample/analyte, dipping sampling end of cotton thread into solvent/mobile phase, running solvent/mobile phase at predetermine time to separate sample; analyzing sample run on cotton thread through visual inspection
2024-12-22
专利权人MAHAJAN S K (MAHA-Individual) ; SONAWANE D D (SONA-Individual) ; SURANA K R (SURA-Individual)
申请日期2024-12-22
专利号IN202421101709-A
成果简介NOVELTY - Performing a cotton thread chromatography comprises: (a) taking a cotton thread, preferably made up of surgical cotton of size of length from 5-10 cm and width from 2-5 mm diameter; (b) spotting the sample/analyte on cotton thread at a distance of 2 cm from one of the end, completely drying the sample/ analyte on thread; (c) tying other end of cotton thread to a holding means preferably of a clamp, fixing the clamp at upper side of chromatographic chamber or flask using clay or any like sticking material; (d) dipping the sampling end of cotton thread into the solvent/ mobile phase 1 cm above from the bottom of chamber/flask and putting a lid on the chamber/flask; (e) running the solvent/ mobile phase at predetermine time to separate the sample; (f) analyzing the sample run on cotton thread through visual inspection, terminating the mobile phase run time, solvent front; removing the holding means of cotton thread from chromatography chamber/flask. USE - Method for performing a cotton thread chromatography. ADVANTAGE - The method utilizes less time and provides accurate & efficient results without employing any manual human efforts in setting up the arrangement of equipment required for performing chromatography, efficient and time effective manner. DETAILED DESCRIPTION - Performing a cotton thread chromatography comprises: (a) taking a cotton thread, preferably made up of surgical cotton of size of length from 5-10 cm and width from 2-5 mm diameter; (b) spotting the sample/analyte on cotton thread at a distance of 2 cm from one of the end, completely drying the sample/ analyte on thread; (c) tying other end of cotton thread to a holding means preferably of a clamp, fixing the clamp at upper side of chromatographic chamber or flask using clay or any like sticking material; (d) dipping the sampling end of cotton thread into the solvent/ mobile phase 1 cm above from the bottom of chamber/flask and putting a lid on the chamber/flask; (e) running the solvent/ mobile phase at predetermine time to separate the sample; (f) analyzing the sample run on cotton thread through visual inspection, terminating the mobile phase run time, solvent front; removing the holding means of cotton thread from chromatography chamber/flask; and removing the cotton thread from holding means and evaluating the retention spots of analytes for further analysis and evaluation to confirm the separated compounds.
IPC 分类号C09B-061/00 ; C09B-067/54 ; G01N-030/06 ; G01N-030/74 ; G01N-030/86
国家印度
专业领域化学化工
语种英语
成果类型专利
文献类型科技成果
条目标识符http://119.78.100.226:8889/handle/3KE4DYBR/13886
专题中国科学院新疆生态与地理研究所
作者单位
1.MAHAJAN S K (MAHA-Individual)
2.SONAWANE D D (SONA-Individual)
3.SURANA K R (SURA-Individual)
推荐引用方式
GB/T 7714
MAHAJAN S K,SONAWANE D D,SURANA K R. Performing cotton thread chromatography, comprises taking cotton thread, spotting sample/analyte, dipping sampling end of cotton thread into solvent/mobile phase, running solvent/mobile phase at predetermine time to separate sample; analyzing sample run on cotton thread through visual inspection. IN202421101709-A[P]. 2024.
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