Portable kit for sample staining in impression cytology, comprises eye drop selected from e.g. xylocaine (lidocaine), glass dropper bottles containing working solutions of essential reagents such as alcohol, oxidizing agent, Schiff reagent, hematoxylin solution, xylene dips, and mounting medium
	BUCH A; PRASAD N; MISHRA N; CHOTALIYA R
	2024-12-27
专利权人	DY PATIL VIDYAPEETH PUNE (DYPA-Non-standard)
申请日期	2024-12-27
专利号	IN202421103735-A
成果简介	NOVELTY - Portable kit comprises an eye drop selected from such as xylocaine (lidocaine), proparacaine, and lignocaine, preferably 0.5% proparacaine; a plural of glass dropper bottles of 2-6 ml capacity, containing working solutions of essential reagents including alcohol, 1 % oxidizing agent, Schiff reagent, hematoxylin solution, xylene dips, and mounting medium; self-standing centrifuge tubes of 50-100 ml capacity having screw caps to aliquot the working solutions of the essential reagents, for performing staining process; sterilized filter paper strip/ block to collect test sample for staining; sterilized 2-5 earbuds for pressing the filter paper strip/block and collecting the test sample; sterilized metallic forceps having blunt and round tip for picking and holding the sterilized filter paper strip/ block after collecting the test sample on it; and a manual guide having instructions for using the kit. USE - Portable kit for sample staining in impression cytology. ADVANTAGE - The kit provides quick examination of cells of interest, specifically ocular cells; and is cost-effective, and time-saving. The method comprises minimal steps that provide complete staining of collected cells sample in just 35 minutes after sampling; and is easy, and concise. DETAILED DESCRIPTION - Portable kit comprises an eye drop selected from such as xylocaine (lidocaine), proparacaine, and lignocaine, preferably 0.5% proparacaine; a plural of glass dropper bottles of 2-6 ml capacity, containing working solutions of essential reagents including alcohol, 1 % oxidizing agent, Schiff reagent, hematoxylin solution, xylene dips, and mounting medium; self-standing centrifuge tubes of 50-100 ml capacity having screw caps to aliquot the working solutions of the essential reagents, for performing staining process; sterilized filter paper strip/ block to collect test sample for staining; sterilized 2-5 earbuds for pressing the filter paper strip/block and collecting the test sample; sterilized metallic forceps having blunt and round tip for picking and holding the sterilized filter paper strip/ block after collecting the test sample on it; sterilized 2-5 glass slides and 5-10 cover slips for mounting the test sample on it after staining, for further visualization; a glass dropper for dispensing desired solution(s) for mounting the stained cells on one glass slide; and a manual guide having instructions for using the kit. An INDEPENDENT CLAIM is included for a method for staining and visualizing stained cells which involves: (a) adding 1-2 drops of eye drop; (b) swiping a sterilized filter paper strip/block gently on anesthetized area under examination to collect cells sample using sterilized earbud; (c) holding the filter paper strip/block which contains the collected cells sample, with sterilized metallic forceps and immersing it in 90-97% alcohol for 5-20 minutes to fix the collected cells sample on the filter paper strip / block; (d) immersing the filter paper strip/block containing the fixed cells sample in 65-80% alcohol for 1-5 minute to rehydrate the fixed cells sample on the filter paper strip/block; (e) immersing the filter paper strip/block containing the rehydrated cells sample in 0.5-2% periodic acid solution for 1-5 minutes for oxidation of glycol groups present in the cells sample to aldehydes; (f) washing the filter paper strip/block containing the oxidized cells sample in tap water for 15-40 seconds to remove traces of periodic acid; (g) adding 1 ml Schiff reagent for 5-15 minutes to the washed filer paper strip / block followed by washing it in running tap water for 45-90 seconds; (h) dipping the Schiff- stained and washed filter paper strip/block in 1 ml Harris hematoxylin solution for 30-60 seconds for staining the nucleus followed by washing in running tap water for 15-50 seconds, to obtain filter paper strip/block containing completely stained cells sample; (i) keeping the filter paper strip/block containing the completely stained cells sample in 90-97% alcohol for 1-5 minutes followed by dipping it in absolute ethanol for 1-5 minutes with stirring in between, to remove excess stain to obtain filter paper strip/block containing dehydrated cells sample; (j) exposing the dehydrated cells sample on filter paper strip/block to 1 ml xylene to make the filter paper strip/block transparent and to obtain optimally stained cells sample for proper evaluation; (k) keeping the filter paper strip/block containing the optimally stained cells sample on a slide followed by evaporating the xylene; (l) adding dibutyl phthalate polystyrene xylene on the filter paper strip/block containing optimally stained cells sample; and (m) gently applying cover slip on the top, to prepare a slide for visualization and analysis of the optimally stained cells initially collected from the anesthetized area under examination.
IPC 分类号	G01N-001/28 ; G01N-001/30 ; G01N-033/531 ; G01N-033/539
国家	印度
专业领域	化学化工
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/13778
专题	中国科学院新疆生态与地理研究所
作者单位	DY PATIL VIDYAPEETH PUNE (DYPA-Non-standard)
推荐引用方式 GB/T 7714	BUCH A,PRASAD N,MISHRA N,et al. Portable kit for sample staining in impression cytology, comprises eye drop selected from e.g. xylocaine (lidocaine), glass dropper bottles containing working solutions of essential reagents such as alcohol, oxidizing agent, Schiff reagent, hematoxylin solution, xylene dips, and mounting medium. IN202421103735-A[P]. 2024.

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