Determining optical absorbance of a blood sample used for identifying presence or absence of sickle hemoglobin in blood, involves measuring first absorbance of blood sample under deoxygenated condition followed by subtracting second absorbance from first absorbance to obtain absorbance difference
	SAI S G; JAGANNADH V K; SRUTHI K; VERMA N; SRINIVASAN R; NATH B
	2024-12-31
专利权人	SHANMUKHA INNOVATIONS PRIVATE LTD (SHAN-Non-standard) ; INDIAN INST SCI (INDF-C)
申请日期	2024-12-31
专利号	IN202441105232-A
成果简介	NOVELTY - Determining optical absorbance of a blood sample, involves: (a) measuring a first absorbance of blood sample under a deoxygenated condition at 420-440 nanometers (nm); (b) measuring a second absorbance of blood sample under the deoxygenated condition at 541-570 nm; (c) calculating a ratio of the second absorbance to the first absorbance to obtain an absorbance ratio; and (d) subtracting the second absorbance from the first absorbance to obtain an absorbance difference. USE - Method for determining optical absorbance of a blood sample used for identifying the presence or absence of sickle hemoglobin in a blood sample (claimed). ADVANTAGE - The method is simple, low cost, rapid, and improves accuracy by substantially reducing errors in the identification of the presence or absence of sickle hemoglobin in a sample. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: 1. a kit for performing the method, which comprises: buffer or components; lysing agent; reducing agent; and document comprising instructions to perform the method and a table providing a standard curve based on the absorbance ratio and the absorbance difference; 2. a device for performing the method, which comprises: (a) first light source configured to emit light having a wavelength of 420-440 nm; (b) a second light source configured to emit light having a wavelength of 541-570 nm; (c) a sample holder facing each of the first light source and the second light source, where each first light source and second light source emits light on blood sample in the sample holder; and (d) a detector facing the sample holder, and detector configured to detect light transmitted by the blood sample; and 3. a method of identifying the presence or absence of sickle hemoglobin in a blood sample, which involves: (a) measuring a first absorbance of blood sample under a deoxygenated condition at 420-440 nm; (b) measuring a second absorbance of blood sample under the deoxygenated condition at 541-570 nm; (c) calculating a ratio of the second absorbance to the first absorbance to obtain an absorbance ratio; (d) subtracting the second absorbance from the first absorbance to obtain an absorbance difference; and (e) identifying the presence or absence of sickle hemoglobin based on absorbance ratio and the absorbance difference; and 4. a method of determining a percentage of sickle hemoglobin in a blood sample, which involves: (i) subtracting the second absorbance from the first absorbance to obtain an absorbance difference; and (ii) determining the percentage of sickle hemoglobin in the blood sample based on the absorbance ratio and the absorbance difference.
IPC 分类号	G01N-021/31 ; G01N-033/487 ; G01N-033/49 ; G01N-033/72
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/13650
专题	中国科学院新疆生态与地理研究所
作者单位	1.SHANMUKHA INNOVATIONS PRIVATE LTD (SHAN-Non-standard) 2.INDIAN INST SCI (INDF-C)
推荐引用方式 GB/T 7714	SAI S G,JAGANNADH V K,SRUTHI K,et al. Determining optical absorbance of a blood sample used for identifying presence or absence of sickle hemoglobin in blood, involves measuring first absorbance of blood sample under deoxygenated condition followed by subtracting second absorbance from first absorbance to obtain absorbance difference. IN202441105232-A[P]. 2024.

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